Phospho-PKC Alpha (Thr638) Polyclonal antibody

Phospho-PKC Alpha (Thr638) Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 29123-1-AP

Synonyms

AAG6, Phospho-PKC Alpha (Thr638), PKC A, PKC alpha, PKCA, PRKACA, PRKCA, Protein kinase C alpha type, protein kinase C, alpha

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Validation Data Gallery

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  WB analysis using 29123-1-AP

  Non-treated NIH/3T3, phosphatase inhibitor treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29123-1-AP (Phospho-PKC Alpha (Thr638) antibody) at dilution of 1:5000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  WB analysis using 29123-1-AP

  Non-treated and PMA treated HL-60 cells were subjected to SDS PAGE followed by western blot with 29123-1-AP (Phospho-PKC Alpha (Thr638) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 29123-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed λ phosphatase treated HeLa cells using Phospho-PKC Alpha (Thr638) antibody (29123-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: λ phosphatase treated NIH/3T3 cells, PMA treated HL-60 cells
• Positive IF/ICC detected in: λ phosphatase treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below

Product Information

29123-1-AP targets Phospho-PKC Alpha (Thr638) in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: protein kinase C, alpha
• Calculated Molecular Weight: 77 kDa
• Observed Molecular Weight: 77 kDa
• GenBank Accession Number: AK055431
• Gene Symbol: PKC Alpha
• Gene ID (NCBI): 5578
• RRID: AB_2918239
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PKCs are a family of serine/threonine kinases involved in various processes in cells including proliferation, differentiation, cell survival, and apoptosis. PKC family is composed of three different subgroups: conventional (cPKC), the novel (nPKC) and atypical (aPKC). PKCα, β1, β2, and γ belong to cPKC, PKCδ, ε, η and θ are nPKC, whereas aPKC comprises of PKCζ and λ/ι. All PKCs consist of the N-terminal regulatory region and C-terminal catalytic region (kinase domain). PKCs are physiologically activated by various extracellular signals transduced by hormones, growth factors, cytokines or antigens. The presence of activated PKCs on internal membranes leads to the phosphorylation of various interacting proteins. (PMID: 32466765, PMID: 12417016)

Publications

Species	Application	Title
human	WB	Chem Biol Interact; Lead-induced cardiomyocytes apoptosis by inhibiting gap junction intercellular communication via modulating the PKCα/Cx43 signaling pathway; Authors - Qiong Wang
human	WB	Cancer Med; MAPK4 predicts poor prognosis and facilitates the proliferation and migration of glioma through the AKT/mTOR pathway; Authors - Jing Ren
mouse	WB	J Leukoc Biol; Transient receptor potential vanilloid 4 (TRPV4) in neutrophils enhances myocardial ischemia/reperfusion injury; Authors - Yuwei Wu
mouse	WB	Int J Mol Sci; Oryza sativa L. Indica Seed Coat Ameliorated Concanavalin A-Induced Acute Hepatitis in Mice via MDM2/p53 and PKCα/MAPK1 Signaling Pathways; Authors - Zhiye Zhao
human	WB	Cell Rep Med; Cholinergic signaling via muscarinic M1 receptor confers resistance to docetaxel in prostate cancer; Authors - Jing Wang