Phospho-PERK/EIF2AK3 (Ser719) Polyclonal antibody

Phospho-PERK/EIF2AK3 (Ser719) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (5)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 29546-1-AP

Synonyms

DKFZp781H1925, EIF2AK3, HRI, HsPEK, Pancreatic eIF2 alpha kinase, PEK, PERK, Phospho-PERK/EIF2AK3 (Ser719), WRS

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Validation Data Gallery

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  WB analysis using 29546-1-AP

  Non-treated HeLa cells, phosphatase inhibitor treated and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 29546-1-AP (Phospho-PERK/EIF2AK3 (Ser719) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

• 

  WB analysis using 29546-1-AP

  Non-treated HeLa cells and Thapsigargin treated HeLa cells were subjected to SDS PAGE followed by western blot with 29546-1-AP (Phospho-PERK/EIF2AK3 (Ser719) antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: λ phosphatase treated HeLa cells, Thapsigargin treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 18 publications below

Product Information

29546-1-AP targets Phospho-PERK/EIF2AK3 (Ser719) in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse, rat, zebrafish, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: eukaryotic translation initiation factor 2-alpha kinase 3
• Calculated Molecular Weight: 1116 aa, 125 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC126354
• Gene Symbol: PERK
• Gene ID (NCBI): 9451
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570)

Publications

Species	Application	Title
human	WB	Mar Drugs; Ascomylactam C Induces an Immunogenic Cell Death Signature via Mitochondria-Associated ER Stress in Lung Cancer and Melanoma; Authors - Yun Huang
human	WB	World J Gastroenterol; Cryptotanshinone induces apoptosis of activated hepatic stellate cells via modulating endoplasmic reticulum stress; Authors - Xiao-Xue Hou
mouse,rat	WB	Prostaglandins Other Lipid Mediat; Dahuang Huanglian Xiexin Decoction ameliorates obesity via modulating adipocyte differentiation and lipid degradation through inhibiting endoplasmic reticulum stress; Authors - Long Hao
rat	WB	Pharmaceuticals (Basel); Maltol Improves Peripheral Nerve Function by Inhibiting Schwann Cell Apoptosis via the PERK/eIF2α/CHOP Pathway and MME Upregulation in Diabetic Peripheral Neuropathy; Authors - Jiawei Li
mouse	WB	Ecotoxicol Environ Saf; Endoplasmic reticulum stress promotes oxidative stress, inflammation, and apoptosis: A novel mechanism of citrinin-induced renal injury and dysfunction; Authors - Yongkang Wang
mouse	WB	Am J Physiol Heart Circ Physiol; One day of environment-induced heat stress damages the murine myocardium; Authors - Melissa Roths