Phospho-PERK/EIF2AK3 (Ser719) Polyclonal antibody

Phospho-PERK/EIF2AK3 (Ser719) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29546-1-AP

Synonyms

EIF2AK3, PERK, Pancreatic eIF2 alpha kinase, HsPEK, Eukaryotic translation initiation factor 2-alpha kinase 3



Tested Applications

Positive WB detected inλ phosphatase treated HeLa cells, Thapsigargin treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 23 publications below

Product Information

29546-1-AP targets Phospho-PERK/EIF2AK3 (Ser719) in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, zebrafish, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name eukaryotic translation initiation factor 2-alpha kinase 3
Calculated Molecular Weight 1116 aa, 125 kDa
Observed Molecular Weight140 kDa
GenBank Accession NumberBC126354
Gene Symbol PERK
Gene ID (NCBI) 9451
RRIDAB_2935416
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570)

Protocols

Product Specific Protocols
WB protocol for Phospho-PERK/EIF2AK3 (Ser719) antibody 29546-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mar Drugs

Ascomylactam C Induces an Immunogenic Cell Death Signature via Mitochondria-Associated ER Stress in Lung Cancer and Melanoma

Authors - Yun Huang
humanWB

World J Gastroenterol

Cryptotanshinone induces apoptosis of activated hepatic stellate cells via modulating endoplasmic reticulum stress

Authors - Xiao-Xue Hou
mouse,ratWB

Prostaglandins Other Lipid Mediat

Dahuang Huanglian Xiexin Decoction ameliorates obesity via modulating adipocyte differentiation and lipid degradation through inhibiting endoplasmic reticulum stress

Authors - Long Hao
ratWB

Pharmaceuticals (Basel)

Maltol Improves Peripheral Nerve Function by Inhibiting Schwann Cell Apoptosis via the PERK/eIF2α/CHOP Pathway and MME Upregulation in Diabetic Peripheral Neuropathy

Authors - Jiawei Li
mouseWB

Ecotoxicol Environ Saf

Endoplasmic reticulum stress promotes oxidative stress, inflammation, and apoptosis: A novel mechanism of citrinin-induced renal injury and dysfunction

Authors - Yongkang Wang
mouseWB

Am J Physiol Heart Circ Physiol

One day of environment-induced heat stress damages the murine myocardium

Authors - Melissa Roths