Phospho-PDH E1 Alpha (Ser300) Recombinant antibody

• 

  WB analysis using 80572-1-RR

  Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 80572-1-RR (Phospho-PDH E1 Alpha (Ser300) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control.

• 

  WB analysis of HSC-T6 using 80572-1-RR

  Non-treated and Calyculin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 80572-1-RR (Phospho-PDH E1 Alpha (Ser300) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control.

• 

  WB analysis of NIH/3T3 using 80572-1-RR

  Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80572-1-RR (Phospho-PDH E1 Alpha (Ser300) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control.

• 

  IF Staining of HepG2 using 80572-1-RR

  Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HepG2 cells using Phospho-PDH E1 Alpha (Ser300) antibody (80572-1-RR, Clone: 2E19 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Phospho-PDH E1 Alpha (Ser300) {ptg:Brand} Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 80572-1-RR

Clone No.2E19

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Synonyms

p PDH E1 Alpha, p PDH E1 Alpha (Ser300), p PDH E1 Alpha Ser300, PDH E1 Alpha (Ser300), Phospho PDH E1 Alpha

---

Tested Applications

• Positive WB detected in: HeLa cells, HSC-T6 cells, NIH/3T3 cells, Calyculin A treated HeLa cells, Calyculin A treated HEK-293 cells, Calyculin A treated HSC-T6 cells, Calyculin A treated NIH/3T3 cells
• Positive IF/ICC detected in: λ phosphatase treated HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IP: See 1 publications below

Product Information

80572-1-RR targets Phospho-PDH E1 Alpha (Ser300) in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: pyruvate dehydrogenase (lipoamide) alpha 1
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC002406
• Gene Symbol: PDHA1
• Gene ID (NCBI): 5160
• RRID: AB_2918903
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08559
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDH E1 Alpha (PDHA1), as the major component of PDH, can be phosphorylated and inactivated by PDHK1. PDHA1 contains three serine residues (Ser232, Ser293, or Ser300) that can be reversibly phosphorylated by a dedicated family of four inhibitory pyruvate dehydrogenase kinases (PDHK1-4) and two reactivating phosphatases (PDP1, 2). Hypoxia induces the expression of PDHK1 and PDHK3 and hyperphosphorylates PDHA1. Phosphorylated PDHA1 inactivates the whole pyruvate dehydrogenase complex (PDC), reduces pyruvate entering into the tricarboxylic acid (TCA) cycle for oxidative phosphorylation, enhances the Warburg effect and promotes tumorigenesis. (PMID: 30993888, PMID: 34749809)

Publications

Species	Application	Title
	WB,IP	Science; Boosting neuronal activity-driven mitochondrial DNA transcription improves cognition in aged mice; Authors - Wenwen Li
human,mouse	WB	Nat Commun; NOTCH1 mitochondria localization during heart development promotes mitochondrial metabolism and the endothelial-to-mesenchymal transition in mice; Authors - Jie Wang