Phospho-PAK1 (Ser144)/PAK2 (Ser141) Recombinant antibody, PBS Only

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  WB analysis using 85044-1-RR (same clone as 85044-1-PBS)

  Non-treated RAW 264.7 cells and LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 85044-1-RR (Phospho-PAK1 (Ser144)/PAK2 (Ser141) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 85044-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 85044-1-RR (same clone as 85044-1-PBS)

  Non-treated C6 cells and pervanadate treated C6 cells were subjected to SDS PAGE followed by western blot with 85044-1-RR (Phospho-PAK1 (Ser144)/PAK2 (Ser141) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 85044-1-PBS in a different storage buffer formulation.

Phospho-PAK1 (Ser144)/PAK2 (Ser141) Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Cat No. 85044-1-PBS

Clone No.242123D12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

PAK1, p21-activated kinase 1, p21 activated kinase 1, EC:2.7.11.1, Alpha-PAK

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Product Information

85044-1-PBS targets Phospho-PAK1 (Ser144)/PAK2 (Ser141) in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: p21 protein (Cdc42/Rac)-activated kinase 1
• Calculated Molecular Weight: 553 aa, 62 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC109299
• Gene Symbol: PAK1
• Gene ID (NCBI): 5058
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q13153
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The human PAK family is divided into the group I (PAK1 to PAK3) and group II (PAK4 to PAK6). Group I PAK share some domains that are not present in the group II members. In particular, the autoinhibitory domain (AID) is important for regulation of the kinase activity of the group I family members. Autophosphorylation at PAK1 Ser144, or at the equivalent sites for the other PAK, stabilizes the open conformation and sustains high kinase activity. Mutation of tyrosines 131 or 429 is associated with reduced dimerization and enhanced kinase activity. (PMID: 31748572)