Phospho-P53 (Ser46) Polyclonal antibody

Phospho-P53 (Ser46) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 28960-1-AP

Synonyms

Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phospho-P53 (Ser46), Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53

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Validation Data Gallery

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  WB analysis using 28960-1-AP

  Non-treated HT-29, phosphatase inhibitor treated and λ phosphatase treated HT-29 cells were subjected to SDS PAGE followed by western blot with 28960-1-AP (Phospho-P53 (Ser46) antibody) at dilution of 1:2000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  WB analysis using 28960-1-AP

  Non-treated and etoposide treated HT-29 cells were subjected to SDS PAGE followed by western blot with 28959-1-AP (Phospho-P53 (Ser46) antibody) at dilution of 1:2000 incubated at room temperature for 1 hours.

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Tested Applications

• Positive WB detected in: HT-29 cells, λ phosphatase treated HT-29 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below

Product Information

28960-1-AP targets Phospho-P53 (Ser46) in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: tumor protein p53
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC003596
• Gene Symbol: TP53
• Gene ID (NCBI): 7157
• RRID: AB_2918223
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. Phosphorylation of p53 at Ser46 regulates the ability of p53 to induce apoptosis. Homeodomain-interacting protein kinase 2 (HIPK2) Phosphorylates Ser46 off p53 and p38 can phosphorylate this site .

Publications

Species	Application	Title
human	WB	Apoptosis; High-dose copper activates p53-independent apoptosis through the induction of nucleolar stress in human cell lines.; Authors - Chieh-Hsin Chen
human	WB	Exp Cell Res; RPS14 promotes the development and progression of glioma via p53 signaling pathway; Authors - Shangwei Hu
human	WB	Mol Cell Probes; Long noncoding RNA RMRP ameliorates doxorubicin-induced apoptosis by interacting with PFN1 in a P53-Dependent manner; Authors - Juexing Li
human	WB	Tissue Cell; DEPDC1B enhances malignant phenotypes of multiple myeloma through upregulating CCNB1 and inhibiting p53 signaling pathway; Authors - Hairong Fei
human	WB	Cell Biol Int; LRP8 promotes tumorigenesis in ovarian cancer through inhibiting p53 signaling; Authors - Yan Xu