Phospho-P53 (Ser46) Polyclonal antibody

Phospho-P53 (Ser46) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG


Human and More (1)





Cat no : 28960-1-AP


Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phospho-P53 (Ser46), Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53

Tested Applications

Positive WB detected inHT-29 cells, λ phosphatase treated HT-29 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 5 publications below

Product Information

28960-1-AP targets Phospho-P53 (Ser46) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name tumor protein p53
Calculated Molecular Weight 44 kDa
Observed Molecular Weight53 kDa
GenBank Accession NumberBC003596
Gene Symbol TP53
Gene ID (NCBI) 7157
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. Phosphorylation of p53 at Ser46 regulates the ability of p53 to induce apoptosis. Homeodomain-interacting protein kinase 2 (HIPK2) Phosphorylates Ser46 off p53 and p38 can phosphorylate this site .


Product Specific Protocols
WB protocol for Phospho-P53 (Ser46) antibody 28960-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




High-dose copper activates p53-independent apoptosis through the induction of nucleolar stress in human cell lines.

Authors - Chieh-Hsin Chen

Exp Cell Res

RPS14 promotes the development and progression of glioma via p53 signaling pathway

Authors - Shangwei Hu

Mol Cell Probes

Long noncoding RNA RMRP ameliorates doxorubicin-induced apoptosis by interacting with PFN1 in a P53-Dependent manner

Authors - Juexing Li

Tissue Cell

DEPDC1B enhances malignant phenotypes of multiple myeloma through upregulating CCNB1 and inhibiting p53 signaling pathway

Authors - Hairong Fei

Cell Biol Int

LRP8 promotes tumorigenesis in ovarian cancer through inhibiting p53 signaling

Authors - Yan Xu