Phospho-P53 (Ser15) Polyclonal antibody

Phospho-P53 (Ser15) Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

26

Cat no : 28961-1-AP

Synonyms

TP53, P53, Tumour Protein p53, Tumor suppressor p53, tumor protein p53

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Validation Data Gallery

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  WB analysis using 28961-1-AP

  Non-treated HT-29 and etoposide treated HT-29 cells were subjected to SDS PAGE followed by western blot with 28961-1-AP (Phospho-P53(Ser15) antibody) at dilution of 1:2000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  IF Staining of HT-29 using 28961-1-AP

  Immunofluorescent analysis of (4% PFA) fixed non-treated HT-29 and etoposide treated HT-29 cells using Phospho-P53 (Ser15) antibody (28961-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HT-29 using 28961-1-AP

  Immunofluorescent analysis of (4% PFA) fixed etoposide treated HT-29 cells using Phospho-P53 (Ser15) antibody (28961-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HT-29 using 28961-1-AP

  Immunofluorescent analysis of (4% PFA) fixed etoposide treated HT-29 cells using Phospho-P53 (Ser15) antibody (28961-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: etoposide treated HT-29 cells
• Positive IF/ICC detected in: etoposide treated HT-29 cells, HT-29 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 24 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

28961-1-AP targets Phospho-P53 (Ser15) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: tumor protein p53
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC003596
• Gene Symbol: P53
• Gene ID (NCBI): 7157
• RRID: AB_2881236
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis . DNA damage induces phosphorylation of p53 at Ser15 and Ser20 and leads to a reduced interaction between p53 and its negative regulator, the oncoprotein MDM2. p53 can be phosphorylated by ATM, ATR, and DNA-PK at Ser15 and Ser37. Phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and activation of p53 in response to DNA damage.

Publications

Species	Application	Title
human	WB,IHC	Adv Sci (Weinh); Transgelin Promotes Glioblastoma Stem Cell Hypoxic Responses and Maintenance Through p53 Acetylation; Authors - Huan Li
mouse	IF	Acta Pharmacol Sin; Exosomes are involved in total body irradiation-induced intestinal injury in mice.; Authors - Hang Li
human	WB	J Nutr Biochem; Lycopene suppresses gastric cancer cell growth without affecting normal gastric epithelial cells; Authors - Ying Zhou
human	WB	Clin Epigenetics; Comprehensive comparison between azacytidine and decitabine treatment in an acute myeloid leukemia cell line; Authors - Tina Aumer
human	WB	Apoptosis; High-dose copper activates p53-independent apoptosis through the induction of nucleolar stress in human cell lines.; Authors - Chieh-Hsin Chen
	WB	J Biol Chem; Vibrio splendidus flagellin C binds tropomodulin to induce p38 MAPK-mediated p53-dependent coelomocyte apoptosis in Echinodermata.; Authors - Fa Dai