Phospho-eNOS (Thr495) Polyclonal antibody

Phospho-eNOS (Thr495) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28939-1-AP

Synonyms

cNOS, Constitutive NOS, EC NOS, ECNOS, Endothelial NOS, eNOS, NOS type III, NOS3, NOSIII, Phospho-NOS3 (Thr495)



Tested Applications

Positive WB detected inVEGF treated HUVEC cells, EGF treated HUVEC cells, Cobalt Chloride treated HUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 5 publications below

Product Information

28939-1-AP targets Phospho-eNOS (Thr495) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name nitric oxide synthase 3 (endothelial cell)
Calculated Molecular Weight133 kDa
Observed Molecular Weight133 kDa
GenBank Accession NumberBC063294
Gene Symbol eNOS
Gene ID (NCBI) 4846
RRIDAB_2881234
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Endothelial NOS (eNOS), also known as nitric oxide synthase 3 (NOS3), has a protective function in the cardiovascular system, which is attributed to NO production. Polymorphisms in NOS3 gene affects the susceptibility to several diseases such as hypertension, preeclampsia, diabetes mellitus, obesity, erectile dysfunction, and migraine.

Protocols

Product Specific Protocols
WB protocol for Phospho-eNOS (Thr495) antibody 28939-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Hypertension

Statins Prevent the Deleterious Consequences of Placental Chemerin Upregulation in Preeclampsia

Authors - Lunbo Tan
ratWB

J Ethnopharmacol

Dahuang Zhechong pill attenuates hepatic sinusoidal capillarization in liver cirrhosis and hepatocellular carcinoma rat model via MK/integrin signaling pathway

Authors - Chuankui Fu
ratWB

Drug Des Devel Ther

Clopidogrel-Induced Gastric Injury in Rats is Attenuated by Stable Gastric Pentadecapeptide BPC 157.

Authors - Hailu Wu
humanWB

J Oncol

Hax-1 Regulates Radiation-Induced Mitochondrial-Dependent Apoptosis of Uveal Melanoma Cells through PI3K/AKT/eNOS Pathway.

Authors - Sha Wang
humanWB

Cytotherapy

Porous gelatin microspheres implanted with adipose mesenchymal stromal cells promote angiogenesis via protein kinase B/endothelial nitric oxide synthase signaling pathway in bladder reconstruction

Authors - Jun Zhao