Phospho-NDRG1 (Thr346) Polyclonal antibody

Phospho-NDRG1 (Thr346) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30886-1-AP

Synonyms

CAP43, CMT4D, DRG 1, DRG1, GC4, HMSNL, N myc downstream regulated 1, NDR1, NDRG1, Protein NDRG1, PROXY1, RIT42, RTP, TARG1, TDD5

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Validation Data Gallery

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  WB analysis using 30886-1-AP

  Non-treated A431 cells, phosphatase inhibitor treated A431 cells and λ phosphatase treated A431 cells were subjected to SDS PAGE followed by western blot with 30886-1-AP (Phospho-NDRG1 (Thr346) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

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Tested Applications

• Positive WB detected in: A431 cells, λ phosphatase treated A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30886-1-AP targets Phospho-NDRG1 (Thr346) in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: N-myc downstream regulated 1
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC006260
• Gene Symbol: NDRG1
• Gene ID (NCBI): 10397
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

N-Myc Downstream Regulated 1 (NDRG1) is a member of the N-myc down-regulated gene family which belongs to the alpha/beta hydrolase superfamily. The phosphorylation of NDRG1 (on Thr346) showed the greatest statistically significant increase in clear cell renal cell carcinoma (ccRCC) compared with noncancerous renal tissue. Further study showed the phosphorylation of NDRG1 (Thr346) in ccRCC cells was maintained by RICTOR and mTOR in complex 2. (PMID: 37298315)