Phospho-MYL9 (Thr19/Ser20) Polyclonal antibody

Phospho-MYL9 (Thr19/Ser20) Polyclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 29504-1-AP

Synonyms

MYL9, MLC-2C, MLC2

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Validation Data Gallery

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  WB analysis using 29504-1-AP

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 29504-1-AP (Phospho-MYL9 (Thr19/Ser20) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  FC experiment of HeLa using 29504-1-AP

  1x10^6 HeLa cells untreated (dashed lines) or treated with λ phosphatase (red) were intracellularly stained with 0.25 ug Phospho-MYL9 (Thr19/Ser20) Polyclonal antibody (29504-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Tested Applications

• Positive WB detected in: Calyculin A treated HeLa cells
• Positive FC (Intra) detected in: λ phosphatase treated HeLa cells

This antibody, also known as Thr18/Ser19, is the site where the initiator methionine is removed.

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

29504-1-AP targets Phospho-MYL9 (Thr19/Ser20) in WB, IF, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: myosin, light chain 9, regulatory
• Calculated Molecular Weight: 20 kDa
• Observed Molecular Weight: 19-20 kDa
• GenBank Accession Number: BC002648
• Gene Symbol: MYL9
• Gene ID (NCBI): 10398
• RRID: AB_2918318
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myosin regulatory light polypeptide 9 (MYL9), also known as MLC2, belongs to the myosin regulatory subunits. It plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation at Thr19 and Ser20. Implicated in cytokinesis, receptor capping, and cell locomotion (PMID:11942626, PMID:2526655). Some studies have demonstrated that MYL9 may play important roles in various human cancers. The expression and phosphorylation of MYL9 (Thr19/Ser20) may be increased in human breast (PMID: 22144583) and liver cancers (PMID: 18648664), while decreased in human colon (PMID: 22752057) and bladder cancers (PMID: 21139803). MYL9 was the only gene differentially expressed in the aged versus young injured arteries in the rat smooth muscle cell layers (PMID:22003410).

Publications

Species	Application	Title
human	IF	Front Cell Dev Biol; Paracrine HB-EGF signaling reduce enhanced contractile and energetic state of activated decidual fibroblasts by rebalancing SRF-MRTF-TCF transcriptional axis; Authors - Junaid Afzal
human	WB	Int J Mol Sci; Non-Muscle MLCK Contributes to Endothelial Cell Hyper-Proliferation through the ERK Pathway as a Mechanism for Vascular Remodeling in Pulmonary Hypertension; Authors - Mariam Anis
human	WB	FASEB J; Pilocarpine mediated excessive calcium accumulation leads to ciliary muscle cell senescence and apoptosis; Authors - Xiang Gao