Phospho-TAK1 (Thr187) Polyclonal antibody

Phospho-TAK1 (Thr187) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 28958-1-AP

Synonyms

MAP3K7, Phospho-MAP3K7/TAK1 (Thr187), TAK1, TGF beta activated kinase 1, TGF1a

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Validation Data Gallery

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  WB analysis using 28958-1-AP

  Non-treated HEK-293T and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 28958-1-AP (Phospho-TAK1 (Thr187) antibody) at dilution of 1:5000 incubated 4℃ overnight. The membrane was stripped and reblotted with GAPDH antibody as a loading control.

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Tested Applications

• Positive WB detected in: Calyculin A treated HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

28958-1-AP targets Phospho-TAK1 (Thr187) in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: mitogen-activated protein kinase kinase kinase 7
• Calculated Molecular Weight: 579 aa, 64 kDa
• Observed Molecular Weight: 75-85 kDa
• GenBank Accession Number: BC017715
• Gene Symbol: TAK1
• Gene ID (NCBI): 6885
• RRID: AB_2918222
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAP3K7(Mitogen-activated protein kinase kinase kinase 7) is also named as TAK1 and belongs to the MAP kinase kinase kinase subfamily.It plays an important role in the cascades of cellular responses evoked by changes in the environment.It has been linked to interleukin-1 receptor and tumor necrosis factor receptor signaling (PMID: 16186825).It has 4 isoforms (53-55 kDa; 64-70 kDa and 75-80 kDa)produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Mol Cell Proteomics; Global Phosphoproteomics Unveils Kinase-regulated Networks in Systemic Lupus Erythematosus; Authors - Shuhui Meng
human	WB	Leukemia; Thrombin receptor activating peptide-6 decreases acute graft-versus-host disease through activating GPR15; Authors - Cong Liu
human	WB	Cell Mol Immunol; SMAD7 expression in CAR-T cells improves persistence and safety for solid tumors; Authors - Sixin Liang