Phospho-LATS1 (Thr1079) Polyclonal antibody

Phospho-LATS1 (Thr1079) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28998-1-AP

Synonyms

h warts, LATS1, Phospho-LATS1 (Thr1079), WARTS, WARTS protein kinase, wts



Tested Applications

Positive WB detected inCalyculin A treated HeLa cells

The antibody also detects the phosphorylated at Thr1041 of the LATS2.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 6 publications below

Product Information

28998-1-AP targets Phospho-LATS1 (Thr1079) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name LATS, large tumor suppressor, homolog 1 (Drosophila)
Calculated Molecular Weight 15 kDa, 127 kDa
Observed Molecular Weight140-150 kDa
GenBank Accession NumberBC015665
Gene Symbol LATS1
Gene ID (NCBI) 9113
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The large tumour suppressor 1 (LATS1) acting as a tumour suppressor is a serine/threonine kinase of the AGC kinase family and is found to be down-regulated in various types of human cancers. Recently, LATS1 has been identified as a central player of the emerging tumour suppressive Hippo signaling pathway. In this pathway, the MST1/2 kinase phosphorylates activates LATS1/2 which, together with its co-factor MOB1, subsequently phosphorylates and inhibits the YAP transcriptional factor by preventing them from translocating to the nucleus. The antibody also detects the phosphorylated at Thr1041 of the LATS2. (PMID: 32460168, PMID: 30219064,PMID: 31792377)

Protocols

Product Specific Protocols
WB protocol for Phospho-LATS1 (Thr1079) antibody 28998-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Biol Sci

S100 Calcium Binding Protein A16 Promotes Cell Proliferation by triggering LATS1 ubiquitin degradation mediated by CUL4A ligase to inhibit Hippo pathway in Glioma development

Authors - Yifang Hu
humanWB

Cell Mol Biol Lett

KIAA1429-mediated m6A modification of CHST11 promotes progression of diffuse large B-cell lymphoma by regulating Hippo-YAP pathway

Authors - Xiaomin Chen

J Transl Med

Modulation of SRC by SNTB1 activates the Hippo-YAP pathway during colon adenocarcinoma metastasis

Authors - Zhengyan Chang
humanWB

Biochem Biophys Res Commun

IGF2BP1 enhances the stability of SIK2 mRNA through m6A modification to promote non-small cell lung cancer progression

Authors - Yan Xu
humanWB

Exp Eye Res

Targeting mechanics-induced trabecular meshwork dysfunction through YAP-TGFβ Ameliorates high myopia-induced ocular hypertension

Authors - Qianwen Bu
humanWB

Discov Oncol

GALNT2 targeted by miR-139-5p promotes proliferation of clear cell renal cell carcinoma via inhibition of LATS2 activation

Authors - Haisheng Yi