Phospho-LATS1 (Thr1079) Polyclonal antibody

Phospho-LATS1 (Thr1079) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 28998-1-AP

Synonyms

h warts, LATS1, Phospho-LATS1 (Thr1079), WARTS, WARTS protein kinase, wts

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Validation Data Gallery

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  WB analysis using 28998-1-AP

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 28998-1-AP (Phospho-LATS1 (Thr1079) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Calyculin A treated HeLa cells

The antibody also detects the phosphorylated at Thr1041 of the LATS2.

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below

Product Information

28998-1-AP targets Phospho-LATS1 (Thr1079) in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: LATS, large tumor suppressor, homolog 1 (Drosophila)
• Calculated Molecular Weight: 15 kDa, 127 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC015665
• Gene Symbol: LATS1
• Gene ID (NCBI): 9113
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The large tumour suppressor 1 (LATS1) acting as a tumour suppressor is a serine/threonine kinase of the AGC kinase family and is found to be down-regulated in various types of human cancers. Recently, LATS1 has been identified as a central player of the emerging tumour suppressive Hippo signaling pathway. In this pathway, the MST1/2 kinase phosphorylates activates LATS1/2 which, together with its co-factor MOB1, subsequently phosphorylates and inhibits the YAP transcriptional factor by preventing them from translocating to the nucleus. The antibody also detects the phosphorylated at Thr1041 of the LATS2. (PMID: 32460168, PMID: 30219064,PMID: 31792377)

Publications

Species	Application	Title
human	WB	Int J Biol Sci; S100 Calcium Binding Protein A16 Promotes Cell Proliferation by triggering LATS1 ubiquitin degradation mediated by CUL4A ligase to inhibit Hippo pathway in Glioma development; Authors - Yifang Hu
human	WB	Cell Mol Biol Lett; KIAA1429-mediated m6A modification of CHST11 promotes progression of diffuse large B-cell lymphoma by regulating Hippo-YAP pathway; Authors - Xiaomin Chen
human	WB	Cell Death Dis; PLAGL2 promotes bladder cancer progression via RACGAP1/RhoA GTPase/YAP1 signaling; Authors - Hualin Chen
human	WB	Biochem Biophys Res Commun; IGF2BP1 enhances the stability of SIK2 mRNA through m6A modification to promote non-small cell lung cancer progression; Authors - Yan Xu
human	WB	Exp Eye Res; Targeting mechanics-induced trabecular meshwork dysfunction through YAP-TGFβ Ameliorates high myopia-induced ocular hypertension; Authors - Qianwen Bu
human	WB	Discov Oncol; GALNT2 targeted by miR-139-5p promotes proliferation of clear cell renal cell carcinoma via inhibition of LATS2 activation; Authors - Haisheng Yi