Phospho-JUN (Ser73) Polyclonal antibody

Phospho-JUN (Ser73) Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (2)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28891-1-AP

Synonyms

Activator protein 1, AP 1, AP1, c Jun, JUN, jun oncogene, P39, Phospho-JUN (Ser73), Proto oncogene c Jun, Transcription factor AP 1



Tested Applications

Positive WB detected inUV treated NIH/3T3 cells
Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28891-1-AP targets Phospho-JUN (Ser73) in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name jun oncogene
Calculated Molecular Weight 331 aa, 36 kDa
Observed Molecular Weight38-45 kDa
GenBank Accession NumberBC068522
Gene Symbol JUN
Gene ID (NCBI) 3725
RRIDAB_2881228
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

JUN is also named as c-Jun and AP1, belongs to the bZIP family and Jun subfamily. JUN, the most extensively studied protein of the activator protein-1 (AP-1) complex, is involved in numerous cell activities, such as proliferation, apoptosis, survival, tumorigenesis and tissue morphogenesis (PMID: 22180088). JUN is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. JUN is a basic leucine zipper (bZIP) transcription factor that acts as homo- or heterodimer, binding to DNA and regulating gene transcription (PMID: 9732876). In additon, extracellular signals can induce post-translational modifications of JUN, resulting in altered transcriptional activity and target gene expression (PMID:8464713). More over, it has uncovered multiple layers of a complex regulatory scheme in which JUN is able to crosstalk, amplify and integrate different signals for tissue development and disease. Jun is predominantly nuclear, ubiquitinated Jun colocalizes with lysosomal proteins (PMID: 15469925). This antibody is raised against synthetic phosphopeptide corresponding to residues surrounding Ser73 of human JUN, which can detect the bands around 42-45 kDa.

Protocols

Product Specific Protocols
WB protocol for Phospho-JUN (Ser73) antibody 28891-1-APDownload protocol
IF protocol for Phospho-JUN (Ser73) antibody 28891-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB,IF

J Cell Biol

JNK regulates ciliogenesis through the interflagellar transport complex and actin networks

Authors - Maria Chatzifrangkeskou
humanWB

J Cell Mol Med

SIAH2 suppresses c-JUN pathway by promoting the polyubiquitination and degradation of HBx in hepatocellular carcinoma

Authors - Qinghe Hu
mouseWB

Biochem Pharmacol

Activation of CXCL13/CXCR5 axis aggravates experimental autoimmune cystitis and interstitial cystitis/bladder pain syndrome.

Authors - Jiang Zhao
humanWB

Int J Biol Sci

RHOV promotes lung adenocarcinoma cell growth and metastasis through JNK/c-Jun pathway.

Authors - Deyu Zhang
mouseWB

Front Pharmacol

Azithromycin Attenuates Bleomycin-Induced Pulmonary Fibrosis Partly by Inhibiting the Expression of LOX and LOXL-2.

Authors - Xiang Tong
humanWB

Med Oncol

FTO promotes gastric cancer progression by modulating MAP4K4 expression via demethylation in an m6A-dependent manner

Authors - Zhe Yin