Phospho-IRF3 (Ser396) Polyclonal antibody

Phospho-IRF3 (Ser396) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 29528-1-AP

Synonyms

IRF3, IRF-3, IRF 3, interferon regulatory factor 3

---

Validation Data Gallery

• 

  WB analysis using 29528-1-AP

  Untreated and LPS treated THP-1 cells were subjected to SDS PAGE followed by western blot with 29528-1-AP (Phospho-IRF3 (Ser396) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as loading control.

---

Tested Applications

• Positive WB detected in: LPS treated THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 12 publications below
• IHC: See 1 publications below

Product Information

29528-1-AP targets Phospho-IRF3 (Ser396) in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, pig, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: interferon regulatory factor 3
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC009395
• Gene Symbol: IRF3
• Gene ID (NCBI): 3661
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interferons (IFNs) are a large family of multifunctional secreted proteins involved in antiviral defense, cell growth regulation, and immune activation(PMID: 18440553). A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. IRF-3 is activated through phosphorylation by the kinases IKKɛ and/or TBK1. Phosphorylation at Ser396 is essential for IRF-3 activation. After cytoplasmic IRF-3 is phosphorylated at Ser386 and Ser396, phosphorylated IRF-3 translocates into the nucleus. This phosphorylated IRF-3 activates IFN and IFN-responsive genes.

Publications

Species	Application	Title
pig	WB	Int J Biol Macromol; Role of OGDH in Atophagy-IRF3-IFN-β pathway during classical swine fever virus infection; Authors - Sen Zeng
human	WB	Int J Mol Sci; FHL2 Inhibits SARS-CoV-2 Replication by Enhancing IFN-β Expression through Regulating IRF-3; Authors - Zhiqiang Xu
human	WB	J Transl Med; Therapeutic efficacy of a novel self-assembled immunostimulatory siRNA combining apoptosis promotion with RIG-I activation in gliomas; Authors - Junxiao Chen
mouse	WB	Adv Sci (Weinh); T Cell-Derived Apoptotic Extracellular Vesicles Hydrolyze cGAMP to Alleviate Radiation Enteritis via Surface Enzyme ENPP1; Authors - Yang Zhou
human	WB	Vet Microbiol; Hunnivirus structural protein VP2 inhibits beta interferon production by targeting the IRF3 essential modulator; Authors - Xinyue Zhu
rat	WB	Phytomedicine; Troxerutin dampened hypothalamic neuroinflammation via microglial IL-22/IL-22R1/IRF3 activation in dihydrotestosterone-induced polycystic ovary syndrome rats; Authors - Zixuan Gao