Phospho-IRF3 (Ser396) Polyclonal antibody

Phospho-IRF3 (Ser396) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (4)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 29528-1-AP

Synonyms

interferon regulatory factor 3, IRF 3, IRF3, Phospho-IRF3 (Ser396)

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Validation Data Gallery

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  WB analysis using 29528-1-AP

  Untreated and LPS treated THP-1 cells were subjected to SDS PAGE followed by western blot with 29528-1-AP (Phospho-IRF3 (Ser396) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as loading control.

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Tested Applications

• Positive WB detected in: LPS treated THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below

Product Information

29528-1-AP targets Phospho-IRF3 (Ser396) in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse, monkey, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: interferon regulatory factor 3
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC009395
• Gene Symbol: IRF3
• Gene ID (NCBI): 3661
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interferons (IFNs) are a large family of multifunctional secreted proteins involved in antiviral defense, cell growth regulation, and immune activation(PMID: 18440553). A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. IRF-3 is activated through phosphorylation by the kinases IKKɛ and/or TBK1. Phosphorylation at Ser396 is essential for IRF-3 activation. After cytoplasmic IRF-3 is phosphorylated at Ser386 and Ser396, phosphorylated IRF-3 translocates into the nucleus. This phosphorylated IRF-3 activates IFN and IFN-responsive genes.

Publications

Species	Application	Title
pig	WB	Int J Biol Macromol; Role of OGDH in Atophagy-IRF3-IFN-β pathway during classical swine fever virus infection; Authors - Sen Zeng
human	WB	Int J Mol Sci; FHL2 Inhibits SARS-CoV-2 Replication by Enhancing IFN-β Expression through Regulating IRF-3; Authors - Zhiqiang Xu
	WB	Signal Transduct Target Ther; The cGAS-STING pathway-dependent sensing of mitochondrial DNA mediates ocular surface inflammation; Authors - Weijie Ouyang
monkey	WB	Viruses; Naringenin Improves Innate Immune Suppression after PRRSV Infection by Reactivating the RIG-I-MAVS Signaling Pathway, Promoting the Production of IFN-I; Authors - Jiaying Yu
mouse	WB	Inflammopharmacology; Rapamycin can alleviate the submandibular gland pathology of Sjögren's syndrome by limiting the activation of cGAS-STING signaling pathway; Authors - Wen Zhu