Phospho-Histone H2A.X (Ser139) Polyclonal antibody

Phospho-Histone H2A.X (Ser139) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 29380-1-AP

Synonyms

H2A histone family, member X, H2A.X, H2A/X, H2AFX, H2AX, Histone H2A.x, Phospho-Histone H2A.X Ser139, γH2AX

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Validation Data Gallery

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  WB analysis using 29380-1-AP

  Non-treated and UV treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 29380-1-AP (Phospho-Histone H2A.X (Ser139) antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Histone H2A.X antibody and Histone H3 antibody as loading control.

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Tested Applications

• Positive WB detected in: UV treated HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IF: See 1 publications below

Product Information

29380-1-AP targets Phospho-Histone H2A.X (Ser139) in WB, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: H2A histone family, member X
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC013416
• Gene Symbol: Histone H2A.X
• Gene ID (NCBI): 3014
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The histone variant H2AX is a major component of the DNA damage response (DDR), especially functioning in amplifying DNA damage signals. In response to DNA double-strand breaks (DSBs), H2AX is instantaneously phosphorylated at Ser139 (a form called cH2AX) by the kinases ATM and ATR. The phosphorylation of H2AX at Ser139, resulting in the formation of gamma-H2AX puncta in the nuclei, is an early event in the cellular response to DNA damage. Therefore, phospho-Histone H2A. X (Ser139) is also known as γH2AX. The phosphorylation site of H2AX, Ser139, has also been described as Ser140 in other literature, and they recognize the same amino acid site. (PMID: 22908299, PMID: 30106130, PMID:22941631)

Publications

Species	Application	Title
mouse	WB	ACS Nano; Nanointegrative In Situ Reprogramming of Tumor-Intrinsic Lipid Droplet Biogenesis for Low-Dose Radiation-Activated Ferroptosis Immunotherapy; Authors - Qiqi Zhang
mouse	WB	J Nanobiotechnology; Synergistic cerium oxide nanozymes: targeting DNA damage and alleviating tumor hypoxia for improved NSCLC radiotherapy efficiency; Authors - Jie Liu
human	WB	Bioorg Chem; Dual inhibition of oxidative phosphorylation and glycolysis to enhance cancer therapy; Authors - Xi Sheng
mouse	IF	Ecotoxicol Environ Saf; 2-bromoacetamide exposure impairs oocyte maturation in mice and humans primarily via disrupting the cytoskeleton; Authors - Chuanxin Zhang
human	WB	Eur J Med Chem; Leveraging a rationally designed veliparib-based anilide eliciting anti-leukemic effects for the design of pH-responsive polymer nanoformulation; Authors - Amandeep Thakur
human	WB	BMC Pharmacol Toxicol; Panobinostat (LBH589) combined with AM1241 induces cervical cancer cell apoptosis through autophagy pathway; Authors - Bo Sheng