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Phospho-HSP27 (Ser78) Recombinant antibody, PBS Only

Phospho-HSP27 (Ser78) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241528D11

Cat no : 80046-3-PBS

Synonyms

HSP27, HSPB1, Hsp25, HMN2B, Estrogen-regulated 24 kDa protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-HSP27 (Ser78) Recombinant antibody (80046-3-RR)

    WB analysis using 80046-3-RR (same clone as 80046-3-PBS)

    Non treated and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 80046-3-RR (Phospho-HSP27 (Ser78) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80046-3-PBS in a different storage buffer formulation.

Product Information

80046-3-PBS targets Phospho-HSP27 (Ser78) in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name heat shock 27kDa protein 1
Calculated Molecular Weight 19-23 kDa
Observed Molecular Weight25-30 kDa
GenBank Accession NumberBC012768
Gene Symbol HSPB1
Gene ID (NCBI) 3315
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HSPB1, also named as HSP27, belongs to the mammalian small HSP family widely expressed in cells including the cytoplasm and nucleus. It has been reported that HSPB1 plays a key role in stress resistance, actin organization and numerous biological processes. HSPB1 can be phosphorylated in different stimulation such as cytokines, growth factors and peptide hormones on the site of Ser15, Ser78 and Ser82 by MAPKAP2 kinase. The phosphorylation of HSPB1 leads to a decrease in the size of HSPB1 oligomers. HSPB1 is associated with amyotrophic lateral sclerosis (ALS) (PMID: 8542580, 11960785, 10383393, 32231288)