Phospho-HSP27 (Ser78) Polyclonal antibody

Phospho-HSP27 (Ser78) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 28900-1-AP

Synonyms

DMPK binding protein, heat shock 27kDa protein 2, Heat shock protein beta 2, Hs.78846, Hsp B1, HSP27, Phospho-HSP27 (Ser78)

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Validation Data Gallery

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  WB analysis using 28900-1-AP

  Non-treated HeLa and TNF alpha (HZ-1014) treated HeLa cells were subjected to SDS PAGE followed by western blot with 28900-1-AP (Phospho-HSP27 (Ser78) antibody) at dilution of 1:1000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  WB analysis using 28900-1-AP

  Non-treated and Calyculin A treated HeLa cells and PC-3 cells were subjected to SDS PAGE followed by western blot with 28900-1-AP (Phospho-HSP27 (Ser78) antibody) at dilution of 1:3000 incubated at 4℃ overnight.

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Tested Applications

• Positive WB detected in: TNF-alpha treated HeLa cells, Calyculin A treated HeLa cells, Calyculin A treated PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

28900-1-AP targets Phospho-HSP27 (Ser78) in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: heat shock 27kDa protein 1
• Calculated Molecular Weight: 19-23 kDa
• Observed Molecular Weight: 27-30 kDa
• GenBank Accession Number: BC012768
• Gene Symbol: HSPB1
• Gene ID (NCBI): 3315
• RRID: AB_2881230
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSPB1, also named as HSP27, belongs to the mammalian small HSP family widely expressed in cells including the cytoplasm and nucleus. It has been reported that HSPB1 plays a key role in stress resistance, actin organization and numerous biological processes. HSPB1 can be phosphorylated in different stimulation such as cytokines, growth factors and peptide hormones on the site of Ser15, Ser78 and Ser82 by MAPKAP2 kinase. The phosphorylation of HSPB1 leads to a decrease in the size of HSPB1 oligomers. HSPB1 is associated with amyotrophic lateral sclerosis (ALS) (PMID: 8542580, 11960785, 10383393, 32231288)

Publications

Species	Application	Title
human	WB	JOR Spine; Phosphorylated heat shock protein 27 improves the bone formation ability of osteoblasts and bone marrow stem cells from patients with adolescent idiopathic scoliosis; Authors - Sihan He