Phospho-GYS1 (Ser641) Polyclonal antibody, PBS Only

• 

  WB analysis using 28855-1-AP (same clone as 28855-1-PBS)

  Non-treated PC-3 cells, phosphatase inhibitor treated and λ phosphatase PC-3 cells were subjected to SDS PAGE followed by western blot with 28855-1-AP (Phospho-GYS1 (Ser641) antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 28855-1-PBS in a different storage buffer formulation.

• 

  IF Staining of PC-3 using 28855-1-AP (same clone as 28855-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated PC-3 cells using Phospho-GYS1 (Ser641) antibody (28855-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28855-1-PBS in a different storage buffer formulation.

Phospho-GYS1 (Ser641) Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 28855-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

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Product Information

28855-1-PBS targets Phospho-GYS1 (Ser641) in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: glycogen synthase 1 (muscle)
• Calculated Molecular Weight: 84 kDa
• Observed Molecular Weight: 84 kDa
• GenBank Accession Number: BC007688
• Gene Symbol: GYS1
• Gene ID (NCBI): 2997
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P13807
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Glycogen synthase 1 (GYS1, GS) catalyzes the key step of glycogen synthesis and plays an important role in glycogen metabolism in liver and muscle. In kidney tissues, glycogen synthase 1 (GYS1) is the most important rate-limiting enzyme functioning in the last step of glycogen synthesis. Pathologically, its deficiency has been shown to cause muscle glycogen storage disease type 0 and death. Studies of tumors showed that GYS1 was rapidly induced under hypoxic conditions and positively correlated with glycogen accumulation in glioblastoma, breast, and colon cancer cell lines. GYS1 is phosphorylated at nine sites and insulin stimulates dephosphorylation of glycogen synthase. Insulin stimulates dephosphorylation of glycogen synthase via PKB-mediated phosphorylation of GSK3. Phosphorylation of GSK3 decreases kinase activity which will decrease phosphorylation of GS and increase glycogens synthase fractional activity. (PMID: 32802186, PMID: 30443599, PMID: 22232606)