Phospho-GSK3B (Tyr216) Polyclonal antibody

Phospho-GSK3B (Tyr216) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 29125-1-AP

Synonyms

GSK3 beta, GSK3B, GSK3A B, GSK-3 beta, GSK 3 beta

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Validation Data Gallery

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  WB analysis using 29125-1-AP

  Non-treated HEK-293T cells, phosphatase inhibitor treated and λ phosphatase treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 29125-1-AP (Phospho-GSK3B (Tyr216) antibody) at dilution of 1:800 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

• 

  WB analysis using 29125-1-AP

  Non-treated PC-3 cells, phosphatase inhibitor treated and λ phosphatase treated PC-3 cells were subjected to SDS PAGE followed by western blot with 29125-1-AP (Phospho-GSK3B (Tyr216) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: λ phosphatase treated HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 9 publications below
• ELISA: See 1 publications below

Product Information

29125-1-AP targets Phospho-GSK3B (Tyr216) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: glycogen synthase kinase 3 beta
• Calculated Molecular Weight: 433 aa, 48 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC000251
• Gene Symbol: GSK3B
• Gene ID (NCBI): 2932
• RRID: AB_2918240
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glycogen synthase kinase 3 (GSK3) proteins are serine/threonine kinases that were originally identified as key regulatory enzymes in glucose metabolism. There are two isoforms, GSK3 alpha and GSK3 beta, that are encoded by separate genes and that are 85% homologous to each other overall with 95% homology in the kinase domains. PI3K pathway downstream of receptor tyrosine kinase signalling is thought to result in inactivation of GSK3 through phosphorylation of the amino-terminal serine residue (Ser9 in GSK3 beta and Ser21 in GSK3 alpha). GSK3 phosphorylation inhibits its catalytic activity towards its substrates, allowing cytosolic stabilization and nuclear translocation of the transcriptional targets, β-catenin and Nrf2. (PMID: 31354196 , PMID: 25750704)

Publications

Species	Application	Title
mouse	WB	Neurochem Res; Involvement of PI3K/AKT Pathway in the Rapid Antidepressant Effects of Crocetin in Mice with Depression-Like Phenotypes; Authors - Susu Lin
rat	WB	Evid Based Complement Alternat Med; Sufentanil Alleviates Sepsis-Induced Myocardial Injury and Stress Response in Rats through the ERK/GSK-3β Signaling Axis.; Authors - Hongcheng Zang
human	WB	Mar Drugs; Antidiabetic Effect of Collagen Peptides from Harpadon nehereus Bones in Streptozotocin-Induced Diabetes Mice by Regulating Oxidative Stress and Glucose Metabolism; Authors - Qianxia Lin
mouse	WB	Front Aging Neurosci; The γ-Adducin 1-357 fragment promotes tau pathology; Authors - Honglu Yu
mouse	WB	Aging (Albany NY); The Wnt/β-catenin signaling pathway inhibits osteoporosis by regulating the expression of TERT: an in vivo and in vitro study; Authors - Yuanqing Cai
human	WB	Eur J Pharmacol; MiR-29a efficiently suppresses the generation of reactive oxygen species and α-synuclein in a cellular model of Parkinson's disease by potentially targeting GSK-3β; Authors - Ya-Ling Yang