CoraLite®594-conjugated Phospho-GSK3B (Ser9) Monoclonal antibody

Phospho-GSK3B (Ser9) Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1C9E2

Cat no : CL594-67558

Synonyms

GSK3 beta, GSK3B, GSK-3 beta, GSK 3 beta, EC:2.7.11.26

---

Validation Data Gallery

• 

  IF Staining of PC-3 using CL594-67558

  Immunofluorescent analysis of (4% PFA) fixed Calyculin A treated PC-3 cells and non-treated PC-3 cells using CoraLite®594 Phospho-GSK3B (Ser9) antibody (CL594-67558, Clone: 1C9E2 ) at dilution of 1:100.

• 

  FC experiment of PC-3 using CL594-67558

  1X10^6 Calyculin A treated PC-3 cells were intracellularly stained with 0.25 ug CoraLite®594 Anti-Human Phospho-GSK3B (Ser9) (CL594-67558, Clone:1C9E2) (red), or 0.25 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with 80% MeOH.

---

Tested Applications

• Positive IF/ICC detected in: Calyculin A treated PC-3 cells
• Positive FC (Intra) detected in: Calyculin A treated PC-3 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67558 targets Phospho-GSK3B (Ser9) in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: glycogen synthase kinase 3 beta
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: NM_002093
• Gene Symbol: GSK3B
• Gene ID (NCBI): 2932
• RRID: AB_2920159
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .