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WB analysis using 67558-1-Ig (same clone as 67558-1-PBS)
Non-treated and Calyculin A treated cell lysates were subjected to SDS PAGE followed by western blot with 67558-1-Ig (Phospho-GSK3B (Ser9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67558-1-PBS in a different storage buffer formulation.
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Non-treated and Calyculin A treated cell lysates were subjected to SDS PAGE followed by western blot with 67558-1-Ig (Phospho-GSK3B (Ser9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67558-1-PBS in a different storage buffer formulation.
WB analysis using 67558-1-Ig (same clone as 67558-1-PBS)
Non-treated and Calyculin A treated PC-3 were subjected to SDS PAGE followed by western blot with 67558-1-Ig (Phospho-GSK3B (Ser9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH Monoclonal antibody (60004-1-Ig) as loading control. This data was developed using the same antibody clone with 67558-1-PBS in a different storage buffer formulation.
×
Non-treated and Calyculin A treated PC-3 were subjected to SDS PAGE followed by western blot with 67558-1-Ig (Phospho-GSK3B (Ser9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH Monoclonal antibody (60004-1-Ig) as loading control. This data was developed using the same antibody clone with 67558-1-PBS in a different storage buffer formulation.
WB analysis using 67558-1-Ig (same clone as 67558-1-PBS)
Non-treated and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 67558-1-Ig (Phospho-GSK3B (Ser9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH Monoclonal antibody (60004-1-Ig) as loading control. This data was developed using the same antibody clone with 67558-1-PBS in a different storage buffer formulation.
×
Non-treated and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 67558-1-Ig (Phospho-GSK3B (Ser9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH Monoclonal antibody (60004-1-Ig) as loading control. This data was developed using the same antibody clone with 67558-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 67558-1-Ig (same clone as 67558-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67558-1-Ig (GSK3B-phospho-S9 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67558-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 67558-1-Ig (same clone as 67558-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67558-1-Ig (GSK3B-phospho-S9 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67558-1-PBS in a different storage buffer formulation.
IF Staining of PC-3 using 67558-1-Ig (same clone as 67558-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed Calyculin A treated PC-3 cells and non-treated PC-3 cells using Phospho-GSK3B (Ser9) antibody (67558-1-Ig, Clone: 1C9E2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67558-1-PBS in a different storage buffer formulation.

Phospho-GSK3B (Ser9) Monoclonal antibody, PBS Only

Phospho-GSK3B (Ser9) Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 67558-1-PBS

Clone No.1C9E2

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

GSK 3 beta, GSK3A B, GSK3B, GSK3B 216P, GSK3B S9, GSK3B Ser9, gsk3beta, Phospho-GSK3B (Ser9), Ser9

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

67558-1-PBS targets Phospho-GSK3B (Ser9) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity	Human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	glycogen synthase kinase 3 beta
Observed Molecular Weight	48 kDa
GenBank Accession Number	NM_002093
Gene Symbol	GSK3B
Gene ID (NCBI)	2932
RRID	AB_2882772
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P49841
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .