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Phospho-GSK3A (Ser21) Polyclonal antibody, PBS Only

Phospho-GSK3A (Ser21) Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28875-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-GSK3A (Ser21) Polyclonal antibody (28875-1-AP)

    WB analysis using 28875-1-AP (same clone as 28875-1-PBS)

    Non-treated PC-3 and Calyculin A treated treated PC-3 cells were subjected to SDS PAGE followed by western blot with 28875-1-AP (Phospho-GSK3A (Ser21) antibody) at dilution of 1:3000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 28875-1-PBS in a different storage buffer formulation.

Product Information

28875-1-PBS targets Phospho-GSK3A (Ser21) in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name glycogen synthase kinase 3 alpha
Calculated Molecular Weight 483 aa, 51 kDa
Observed Molecular Weight51 kDa
GenBank Accession NumberBC027984
Gene Symbol GSK3A
Gene ID (NCBI) 2931
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GSK3A (glycogen synthase kinase 3 alpha), one of the two functional isoforms encoded by the GSK3 gene, is a serine/threonine protein kinase that is involved in cell-cycle progression, differentiation, and apoptosis (PMID: 27049759). Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin.GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3A and Ser9 of GSK-3B. GSK3A acts as a tumor suppressor and has important roles in acute myeloid leukemia (AML), prostate cancer, and pancreatic cancer (PMID: 22326953, 22539113, 23547054).