Phospho-GRB10 (Ser476) Polyclonal antibody

Phospho-GRB10 (Ser476) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28873-1-AP

Synonyms

Grb 10, GRB IR, GRB10, GRB10 adapter protein, GRBIR, IRBP, KIAA0207, MEG1, Phospho-GRB10 (Ser476), RSS

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Validation Data Gallery

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  WB analysis using 28873-1-AP

  Non-treated NIH/3T3, phosphatase inhibitor treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28873-1-AP (Phospho-GRB10 (Ser476) antibody) at dilution of 1:1000 incubated at 4℃ overnight.

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Tested Applications

• Positive WB detected in: λ phosphatase treated NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28873-1-AP targets Phospho-GRB10 (Ser476) in WB, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: growth factor receptor-bound protein 10
• Calculated Molecular Weight: 536aa,61 kDa; 594aa,67 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC024285
• Gene Symbol: GRB10
• Gene ID (NCBI): 2887
• RRID: AB_2918210
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Growth factor receptor-bound protein 10 (GRB10) is an adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. GRB10 has three consensus domains including pleckstrin homology (PH) domain, SH2/SH3 domain and Ras-associating domain. By binding to kinases, GRB10 suppresses signals from activated receptors tyrosine kinases, including the INSR and INS-like growth factor (IGF1R) receptors. It may play a role in mediating INS-stimulated ubiquitination of INSR, leading to proteasomal degradation. It has been reported that GRB10 is a direct substrate of mechanistic/mammalian target of mTOR and can be phosphorylated at Ser150, Ser428 and Ser476 identified as MAPK-mediated phosphorylation sites. (PMID: 15952796, 24746805, 21659605)