Phospho-FOXO1 (Ser319) Polyclonal antibody

Phospho-FOXO1 (Ser319) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 28757-1-AP

Synonyms

FKH1, FKHR, forkhead box O1, Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma, FOXO1, FOXO1A, Phospho-FOXO1 (Ser319)

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Validation Data Gallery

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  WB analysis using 28757-1-AP

  Non-treated MCF-7 cells and Cobalt Chloride treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 28757-1-AP (Phospho-FOXO1 (Ser319) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control.

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Tested Applications

• Positive WB detected in: MCF-7 cells, Cobalt Chloride treated MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

28757-1-AP targets Phospho-FOXO1 (Ser319) in WB, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: forkhead box O1
• Calculated Molecular Weight: 655 aa, 70 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC021981
• Gene Symbol: FOXO1
• Gene ID (NCBI): 2308
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The forkhead box class O (FoxO) family in mammals is comprised of the evolutionally highly conserved forkhead transcription factors, FoxO1, FoxO3a, FoxO4, and FoxO6. FoxO1 is highly expressed in lymphoid cells, especially in T and B cells. FoxO1 phosphorylation determines the transcriptional activity and cellular localization of FoxO1. Results showed that GLK-IKKβ signaling triggered the nuclear export of FoxO1. (PMID: 35966593)

Publications

Species	Application	Title
human	WB,IF	Parasit Vectors; The effect of Toxoplasma gondii infection on galectin-9 expression in decidual macrophages contributing to dysfunction of decidual NK cells during pregnancy; Authors - Xiao Wang
rat	WB	J Ethnopharmacol; HouShiHeiSan attenuates sarcopenia in Middle Cerebral Artery Occlusion (MCAO) rats; Authors - Hu Qi