Phospho-ErbB3/HER3 (Tyr1289) Recombinant antibody

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  WB analysis using 85053-6-RR

  Non-treated MCF-7 cells and human Neuregulin-1 treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 85053-6-RR (Phospho-ErbB3/HER3 (Tyr1289) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control.

Phospho-ErbB3/HER3 (Tyr1289) Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85053-6-RR

Clone No.250208A12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ErbB3/HER3 (Tyr1289), HER3/ErbB3 (Tyr1289), p ErbB3/HER3 (Tyr1289), p HER3/ErbB3, p-ErbB3/HER3 (Tyr1289)

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Tested Applications

• Positive WB detected in: MCF-7 cells, human Neuregulin-1 treated MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85053-6-RR targets Phospho-ErbB3/HER3 (Tyr1289) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)
• Calculated Molecular Weight: 148 kDa
• Observed Molecular Weight: 185 kDa
• GenBank Accession Number: BC002706
• Gene Symbol: ERBB3
• Gene ID (NCBI): 2065
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P21860
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

V-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (ErbB-3, HER3) is a member of the EGF receptor tyrosine kinase family including EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3), and ErbB-4 (HER4) that are frequently overexpressed in a variety of carcinomas. Unlike EGFR and HER2, their paralogue HER3 does not possess intrinsic kinase activity due to substitutions at critical positions in the kinase domain. To phosphorylate tyrosine residues in the HER3 cytoplasmic tail to recruit and activate intracellular signaling molecules, it must interact with another functional tyrosine kinase. HER3 exhibits this crosstalk preferentially with HER2 and EGFR, with which it can form heterodimers. Ligand-induced hetero-dimerization with EGFR or HER2 promotes HER3-tyrosine phosphorylation at 6 positions (1054, 1197, 1222, 1260, 1276, and 1289) in the C-terminal tail capable of recruiting of the SH2-domain-containing p85 activator of phosphatidyl-inositol-3 kinase (PI3K) and promoting activation of the downstream Akt signaling pathway. (PMID: 35249128)