Phospho-EIF4B (Ser406) Recombinant antibody, PBS Only

• Featured Product
• KD/KO Validated

Phospho-EIF4B (Ser406) Uni-rAb^TM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5G14

Cat no : 80059-1-PBS

Synonyms

EIF4B, PRO1843, eIF-4B, EIF 4B, 5G14

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Validation Data Gallery

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  WB analysis using 80059-1-RR (same clone as 80059-1-PBS)

  Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 80059-1-RR (Phospho-EIF4B (Ser406) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80059-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 80059-1-RR (same clone as 80059-1-PBS)

  WB result of Phospho-EIF4B (Ser406) antibody (80059-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Phospho-EIF4B (Ser406) transfected HeLa cells. This data was developed using the same antibody clone with 80059-1-PBS in a different storage buffer formulation.

• 

  IHC staining of Jurkat using 80059-1-RR (same clone as 80059-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 80059-1-RR (Phospho-EIF4B (Ser406) antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80059-1-PBS in a different storage buffer formulation.

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Product Information

80059-1-PBS targets Phospho-EIF4B (Ser406) in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: eukaryotic translation initiation factor 4B
• Calculated Molecular Weight: 611 aa, 69 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC073154
• Gene Symbol: EIF4B
• Gene ID (NCBI): 1975
• RRID: AB_2918859
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

EIF4B is one of the mammalian eukaryotic initiation factors (eIF) that are required for the ATP-dependent binding of mRNA to the 40 S ribosomal subunit, and the other eIF proteins are EIF4A, EIF4F. eIF4B is involved in translation of numerous proliferative or anti-apoptotic mRNAs with highly structured 5'UTR and subsequently affect cell growth and survival. It was reported that false expression and phosphorylation levels of eIF4B are involved in several tumors including breast cancer, cell lymphoblastic leukemia and diffuse large B-cell lymphoma (PMID: 26848623).