Phospho-EIF2S1 (Ser51) Polyclonal antibody

Phospho-EIF2S1 (Ser51) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (3)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

27

Cat no : 28740-1-AP

Synonyms

EIF 2, eIF 2 alpha, EIF 2A, EIF 2alpha, EIF2, EIF2A, EIF2S1, Phospho-EIF2S1 (Ser51)

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Validation Data Gallery

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  WB analysis using 28740-1-AP

  Non-treated PC-3 and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 28740-1-AP (Phospho-EIF2S1 (Ser51) antibody) at dilution of 1:2000 incubated 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Tested Applications

• Positive WB detected in: Calyculin A treated PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 25 publications below
• IHC: See 3 publications below

Product Information

28740-1-AP targets Phospho-EIF2S1 (Ser51) in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: NM_004094
• Gene Symbol: EIF2S1
• Gene ID (NCBI): 1965
• RRID: AB_2881204
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EIF2S1 is one subunit of the translation initiation factor EIF2, which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51

Publications

Species	Application	Title
human	WB	Cancer Lett; LIMK1 m6A-RNA methylation recognized by YTHDC2 induces 5-FU chemoresistance in colorectal cancer via endoplasmic reticulum stress and stress granule formation; Authors - Lu Chen
mouse,human	WB,IHC	J Mater Chem B; Magnetic hyperthermia induces effective and genuine immunogenic tumor cell death with respect to exogenous heating; Authors - Bin Yan
mouse	WB	Oxid Med Cell Longev; The Dietary Supplement γ-Oryzanol Attenuates Hepatic Ischemia Reperfusion Injury via Inhibiting Endoplasmic Reticulum Stress and HMGB1/NLRP3 Inflammasome.; Authors - Yichao Du
chicken	WB	mBio; Infectious bursal disease virus VP5 triggers host shutoff in a transcription-dependent manner; Authors - Xinxin Niu
human	WB	J Steroid Biochem Mol Biol; OBHSA, a novel selective estrogen receptor degrader, overcomes tamoxifen resistance through cell cycle arrest and unfolded protein response-mediated apoptosis in breast cancer; Authors - Rong Shen
human	WB	Front Cardiovasc Med; Plasma Purification Treatment Relieves the Damage of Hyperlipidemia to PBMCs.; Authors - Xiao Meng Zhang