Phospho-Chk1 (Ser345) Polyclonal antibody

Phospho-Chk1 (Ser345) Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 28803-1-AP

Synonyms

CHEK1, CHK1, CHK1 checkpoint homolog, Chk 1, CHEK 1

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Validation Data Gallery

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  WB analysis using 28803-1-AP

  Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 28803-1-AP (Phospho-Chk1 (Ser345) antibody) at dilution of 1:1000 incubated at room temperature for 1 hours.

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  IF Staining of HeLa using 28803-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Calyculin A treated HeLa cells using Phospho-Chk1 (Ser345) antibody (28803-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Calyculin A treated NIH/3T3 cells, Calyculin A treated HeLa cells
• Positive IF/ICC detected in: Calyculin A treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

28803-1-AP targets Phospho-Chk1 (Ser345) in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: CHK1 checkpoint homolog (S. pombe)
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC004202
• Gene Symbol: Chk1
• Gene ID (NCBI): 1111
• RRID: AB_2918206
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The checkpoint kinase 1 (Chk1) is a conserved kinase that imposes cell cycle delays in response to impediments to DNA replication. Chk1 kinase is downstream of the ATR kinase. ATR phosphorylates Chk1 kinase and other proteins to ensure replication is being blocked as to avoid replication fork collapse and DNA damage. Activation of Chk1 involves phosphorylation at Ser317 and Ser345 by ATM/ATR, followed by autophosphorylation of Ser296. In vitro Chk1 binds to and phosphorylate the dual-specificity protein phosphatases Cdc25A, Cdc25B, and Cdc25C, which control cell cycle transitions by dephosphorylating cyclin-dependent kinases. (PMID:22941630, PMID: 32571801, PMID:19276361)

Publications

Species	Application	Title
human	WB	Transl Oncol; The ATR inhibitor VE-821 increases the sensitivity of gastric cancer cells to cisplatin; Authors - Haochen Su
human	WB	Phytomedicine; Epiberberine induced p53/p21-dependent G2/M cell cycle arrest and cell apoptosis in gastric cancer cells by activating γ-aminobutyric acid receptor- β3; Authors - Mengmeng Li
human	WB	J Exp Clin Cancer Res; HIF-1α-HPRT1 axis promotes tumorigenesis and gefitinib resistance by enhancing purine metabolism in EGFR-mutant lung adenocarcinoma; Authors - Pengyu Geng