Product Information
28807-1-PBS targets Phospho-Chk1 (Ser317) in WB, Indirect ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | CHK1 checkpoint homolog (S. pombe) |
| Calculated Molecular Weight | 54 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC004202 |
| Gene Symbol | Chk1 |
| Gene ID (NCBI) | 1111 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14757 |
| Storage Buffer | PBS only , pH 7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
The checkpoint kinase 1 (Chk1) is a conserved kinase that imposes cell cycle delays in response to impediments to DNA replication. Chk1 kinase is downstream of the ATR kinase. ATR phosphorylates Chk1 kinase and other proteins to ensure replication is being blocked as to avoid replication fork collapse and DNA damage. Activation of Chk1 involves phosphorylation at Ser317 and Ser345 by ATM/ATR, followed by autophosphorylation of Ser296. In vitro Chk1 binds to and phosphorylate the dual-specificity protein phosphatases Cdc25A, Cdc25B, and Cdc25C, which control cell cycle transitions by dephosphorylating cyclin-dependent kinases. (PMID:22941630, PMID: 32571801, PMID:19276361)
