Phospho-CREB1 (Ser133) Polyclonal antibody

Phospho-CREB1 (Ser133) Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

27

Cat no : 28792-1-AP

Synonyms

CREB1, Cyclic AMP-responsive element-binding protein 1, CREB-1, CREB 1, CREB

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Validation Data Gallery

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  WB analysis using 28792-1-AP

  Non-treated and Forskolin treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 28792-1-AP (Phospho-CREB1 (Ser133) antibody) at dilution of 1:4000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

• 

  WB analysis using 28792-1-AP

  Non-treated and Anisomycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 28792-1-AP (Phospho-CREB1 (Ser133) antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 28792-1-AP

  Non-treated and Forskolin treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28792-1-AP (Phospho-CREB1 (Ser133) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 28792-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28792-1-AP (Phospho-CREB1 (Ser133) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Forskolin treated HSC-T6 cells, HeLa cells, NIH/3T3 cells, Forskolin treated NIH/3T3 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 27 publications below
• IF: See 1 publications below

Product Information

28792-1-AP targets Phospho-CREB1 (Ser133) in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: cAMP responsive element binding protein 1
• Calculated Molecular Weight: 341 aa, 35 kDa
• Observed Molecular Weight: 35-46 kDa
• GenBank Accession Number: BC010636
• Gene Symbol: CREB1
• Gene ID (NCBI): 1385
• RRID: AB_2918203
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The cAMP-response element binding protein (CREB) is localized in the nucleus and acts as a transcription factor, which binds to the cAMP response element (CRE) of the promoters of its target genes, upon phosphorylation at Ser133 by different receptor-activated protein kinases, such as protein kinase A (PKA), calmodulin-dependent protein kinase (CaMK), mitogen-activated protein kinases (MAPK), and other kinases. The activity of CREB1 in neurons has been correlated with various intracellular processes, including proliferation, differentiation, survival, long-term synaptic potentiation, neurogenesis, and neuronal plasticity. CREB1 belongs to the CREB/activating transcription factor (ATF) family of transcription factors. The antibody also detects the phosphorylated form of the CREB-related protein ATF1. (PMID: 30214393, PMID: 33917483, PMID: 32605164)

Publications

Species	Application	Title
mouse	WB	Free Radic Biol Med; Reprimo (RPRM) mediates neuronal ferroptosis via CREB-Nrf2/SCD1 pathways in radiation-induced brain injury; Authors - Wenyu Shi
human	WB	J Ethnopharmacol; Yangjing Zhongyu decoction facilitates mitochondrial activity, estrogenesis, and energy metabolism in H2O2-induced human granulosa cell line KGN.; Authors - Jia Liu
mouse	WB	Brain Res Bull; Metabotropic glutamate receptor 5 upregulation of γ-aminobutyric acid transporter 3 expression ameliorates cognitive impairment after traumatic brain injury in mice.; Authors - Peng Xu
human	WB	Int J Mol Sci; Downregulation of IL-8 and IL-10 by the Activation of Ca2+-Activated K+ Channel KCa3.1 in THP-1-Derived M2 Macrophages; Authors - Susumu Ohya
mouse	WB	Redox Biol; PM2.5 increases susceptibility to acute exacerbation of COPD via NOX4/Nrf2 redox imbalance-mediated mitophagy; Authors - Xiaoye Fan
human	WB	Exp Cell Res; Dopamine D1 receptor activation ameliorates ox-LDL-induced endothelial cell senescence via CREB/Nrf2 pathway; Authors - Yiming Zhao