Phospho-Beta Catenin (Ser33) Polyclonal antibody, PBS Only

Phospho-Beta Catenin (Ser33) Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28772-1-PBS

Synonyms

---

Validation Data Gallery

• 

  WB analysis using 28772-1-AP (same clone as 28772-1-PBS)

  Non-treated PC-3 cells and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 28772-1-AP (Phospho-Beta Catenin (Ser33) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 28772-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 28772-1-AP (same clone as 28772-1-PBS)

  Non-treated HeLa cells and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 28772-1-AP (Phospho-Beta Catenin (Ser33) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 28772-1-PBS in a different storage buffer formulation.

---

Product Information

28772-1-PBS targets Phospho-Beta Catenin (Ser33) in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: catenin (cadherin-associated protein), beta 1, 88kDa
• Calculated Molecular Weight: 781 aa, 86 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC058926
• Gene Symbol: Beta Catenin
• Gene ID (NCBI): 1499
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Beta Catenin/β-catenin, also known as CTNNB1, is a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The N-terminal (1-49 amino acids) of β-catenin is the key region for its stability and the degradation by ubiquitin-proteasome system, and phosphorylation of β-catenin at the N-terminal Ser33, Ser37, Thr41, and Ser45 promotes its ubiquitination and degradation (PMID: 31930681). Phosphorylated β-catenin is targeted for ubiquitination-dependent proteolysis.