Phospho-ATF2 (Thr71)/ATF7 (Thr53) Polyclonal antibody

Phospho-ATF2 (Thr71)/ATF7 (Thr53) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28790-1-AP

Synonyms

ATF2, ATF7, CRE BP1, CREB 2, CREB2, CREBP1, HB16, Phospho-ATF2 (Thr71), Phospho-ATF2 (Thr71)/ATF7 (Thr53), Phospho-ATF7 (Thr53), TREB7

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Validation Data Gallery

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  WB analysis using 28790-1-AP

  Non-treated NIH/3T3 cells and Anisomycin treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28790-1-AP (Phospho-ATF2 (Thr71)/ATF7 (Thr53) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Tested Applications

• Positive WB detected in: Anisomycin treated NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28790-1-AP targets Phospho-ATF2 (Thr71)/ATF7 (Thr53) in WB, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: activating transcription factor 2
• Calculated Molecular Weight: 209 aa, 23 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC026175
• Gene Symbol: ATF2
• Gene ID (NCBI): 1386
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATF2, also named as CREB2 and CREBP1, contains one bZIP domain and one C2H2-type zinc finger. It belongs to the bZIP family. ATF2 binds to the cAMP-responsive element(CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. ATF2 binds DNA as a dimer and can form a homodimer in the absence of DNA. It binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity. Stress and growth factors activate ATF2 and ATF7 mainly via sequential phosphorylation of two conserved threonine residues in their activation domain. Distinct protein kinases, among which mitogen-activated protein kinases (MAPK), phosphorylate ATF2 on Thr71 and ATF7 on Thr53, resulting in transcriptional activation. The antibody recognizes ATF2 phosphorylation sites Thr71 and ATF7 phosphorylation sites Thr53.