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Phospho-ASK1 (Ser966) Polyclonal antibody, PBS Only

Phospho-ASK1 (Ser966) Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28846-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-ASK1 (Ser966) Polyclonal antibody (28846-1-AP)

    WB analysis using 28846-1-AP (same clone as 28846-1-PBS)

    Non-treated NIH/3T3, phosphatase inhibitor treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28846-1-AP (Phospho-ASK1 (Ser966) antibody) at dilution of 1:500 incubated at 4℃ overnight. This data was developed using the same antibody clone with 28846-1-PBS in a different storage buffer formulation.

Product Information

28846-1-PBS targets Phospho-ASK1 (Ser966) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name mitogen-activated protein kinase kinase kinase 5
Calculated Molecular Weight 155 kDa
Observed Molecular Weight120 kDa
GenBank Accession NumberBC054503
Gene Symbol ASK1
Gene ID (NCBI) 4217
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ASK1(Apoptosis signal-regulating kinase 1) is also named as MAP3K5, MAPKKK5, MEKK5 and belongs to the MAP kinase kinase kinase subfamily. It is an evolutionarily conserved mitogen activated protein 3-kinase that activates both Jnk and p38 mitogen-activated protein kinases. ASK1 is is activated in response to various cytotoxic stresses including TNF, Fas and reactive oxygen species (ROS) such as H2O2, and activates c-Jun NH 2-terminal kinase (JNK) and p38. The predicted MW of ASK1 is around 155 kDa and the 110-120 kDa fragment has also been reported.