Phospho-ALK (Tyr1604) Polyclonal antibody

Phospho-ALK (Tyr1604) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 30266-1-AP

Synonyms

ALK, ALK tyrosine kinase receptor, Anaplastic lymphoma kinase, CD246, Phospho-ALK (Tyr1604), TFG/ALK

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Validation Data Gallery

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  WB analysis using 30266-1-AP

  Non-treated and PMA treated HL-60 cells were subjected to SDS PAGE followed by western blot with 30266-1-AP (Phospho-ALK (Tyr1604) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as loading control.

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Tested Applications

• Positive WB detected in: PMA treated HL-60 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

30266-1-AP targets Phospho-ALK (Tyr1604) in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: anaplastic lymphoma receptor tyrosine kinase
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC157090
• Gene Symbol: ALK
• Gene ID (NCBI): 238
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALK, also named as CD246, is a receptor tyrosine kinase (RTK) that belongs to the protein kinase superfamily. ALK is usually found in the nervous system and appears to play an important role in the normal development and function of the nervous system. ALK was originally identified as part of the NPM (Nucleophosmin)-ALK oncogenic fusion protein, resulting from the (2;5)(p23;q35) translocation that is frequently associated with anaplastic large-cell lymphoma (ALCL). The human ALK appears to exist as a 140 kDa protein, as well as the 220 kDa full-length ALK species and the 140 kDa ALK species is thought to be the result of a cleavage within the extracellular region of full-length ALK. The 140 kDa ALK protein is phosphorylated in response to activation of ALK(PMID: 19459784).

Publications

Species	Application	Title
human	WB	J Med Chem; Distinct Amino Acid-Based PROTACs Target Oncogenic Kinases for Degradation in Non-Small Cell Lung Cancer (NSCLC); Authors - Jianchao Zhang