Phospho-AKT1 (Ser473) Recombinant antibody, PBS Only

Phospho-AKT1 (Ser473) Uni-rAb^TM Recombinant Antibody for Indirect ELISA, WB

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human

Applications

Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

2M10

Cat no : 80462-1-PBS

Synonyms

AKT pan, AKT 1, AKT (pan), AKT, AKT1

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Validation Data Gallery

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  WB analysis using 80462-1-RR (same clone as 80462-1-PBS)

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 80462-1-RR (Phospho-AKT1 (Ser473) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80462-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 80462-1-RR (same clone as 80462-1-PBS)

  Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80462-1-RR (Phospho-AKT1 (Ser473) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80462-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 80462-1-RR (same clone as 80462-1-PBS)

  Non-treated and IGF1 treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 80462-1-RR (Phospho-AKT1 (Ser473) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80462-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 80462-1-RR (same clone as 80462-1-PBS)

  Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80462-1-RR (Phospho-AKT1 (Ser473) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80462-1-PBS in a different storage buffer formulation.

• 

  FC experiment of NIH/3T3 using 80462-1-RR (same clone as 80462-1-PBS)

  1X10^6 NIH/3T3 cells untreated (dashed line) or treated with Calyculin A (red) were intracellularly stained with 0.5 ug Anti-Human Phospho-AKT1 (Ser473) (80462-1-RR, Clone:2M10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80462-1-PBS in a different storage buffer formulation.

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Product Information

80462-1-PBS targets Phospho-AKT1 (Ser473) in Indirect ELISA, WB applications and shows reactivity with mouse, human samples.

• Tested Reactivity: mouse, human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: v-akt murine thymoma viral oncogene homolog 1
• Observed Molecular Weight: 56-62 kDa
• GenBank Accession Number: NM_005163
• Gene Symbol: AKT1
• Gene ID (NCBI): 207
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

AKT is a serine/threonine kinase and it participates in the key role of the PI3K signaling pathway. Phosphatidylinositol-3 kinase (PI3K) is the key regulator of AKT activation. The recruitment of inactive AKT protein to PIP3-rich areas of the plasma membrane results in a conformational change that exposes the activation loop of AKT. AKT's activating kinase, phosphoinositide-dependent protein kinase (PDK1) , is also recruited to PIP3 microdomains. PDK1 phosphorylates AKT on threonine 308 (Thr308) of the exposed activation loop, activating AKT and leading to a second phosphorylation of AKT at serine 473 (Ser473) by a kinase presumed to be mTORC2 that further potentiates kinase activity. Active AKT will phosphorylate various downstream protein targets that control cell growth and translational control and act to suppress apoptosis. (PMID: 31594388, PMID: 30808672). 80462-1-RR specifically recognizes AKT1 phosphorylated at Ser473.