Phospho-AKT (Thr308) Polyclonal antibody

Phospho-AKT (Thr308) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

21

Cat no : 29163-1-AP

Synonyms

AKT pan, AKT 1, AKT (pan), AKT, AKT1

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Validation Data Gallery

• 

  WB analysis using 29163-1-AP

  Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 29163-1-AP (Phospho-AKT (Thr308) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Calyculin A treated HEK-293 cells, Calyculin A treated HeLa cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 20 publications below
• IHC: See 3 publications below

Product Information

29163-1-AP targets Phospho-AKT (Thr308) in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: v-akt murine thymoma viral oncogene homolog 1
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC000479
• Gene Symbol: AKT1
• Gene ID (NCBI): 207
• RRID: AB_2918241
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AKT is a serine/threonine kinase and it participates in the key role of the PI3K signaling pathway. Phosphatidylinositol-3 kinase (PI3K) is the key regulator of AKT activation. The recruitment of inactive AKT protein to PIP3-rich areas of the plasma membrane results in a conformational change that exposes the activation loop of AKT. AKT's activating kinase, phosphoinositide-dependent protein kinase (PDK1) , is also recruited to PIP3 microdomains. PDK1 phosphorylates AKT on threonine 308 (Thr308) of the exposed activation loop, activating AKT and leading to a second phosphorylation of AKT at serine 473 (Ser473) by a kinase presumed to be mTORC2 that further potentiates kinase activity. Active AKT will phosphorylate various downstream protein targets that control cell growth and translational control and act to suppress apoptosis. (PMID: 31594388, PMID: 30808672)

Publications

Species	Application	Title
	IHC	J Exp Clin Cancer Res; RNA-binding protein CCDC137 activates AKT signaling and promotes hepatocellular carcinoma through a novel non-canonical role of DGCR8 in mRNA localization; Authors - Shuang Tao
mouse	WB	Cell Death Dis; CD226 implicated in Akt-dependent apoptosis of CD4+ T cell contributes to asthmatic pathogenesis; Authors - Yuan Zhang
human	WB	Front Pharmacol; Autophagy Inhibition Enhances the Anti-Tumor Activity of Methylseleninic Acid in Cisplatin-Resistance Human Lung Adenocarcinoma Cells.; Authors - Ming Xin
human	WB	Bioengineered; Circadian Period 2 (Per2) downregulate inhibitor of differentiation 3 (Id3) expression via PTEN/AKT/Smad5 axis to inhibits glioma cell proliferation.; Authors - Yifan Zhang
rat	WB	Physiol Res; 6-Hydroxydopamine-Induced Neurotoxicity in Rat Model of Parkinson's Disease: Is Reversed via Anti-Oxidative Activities of Curcumin and Aerobic Exercise Therapy; Authors - H Sokouti
rat	WB	Biotechnol Lett; BMSCs improve TNBS-induced colitis in rats by inducing Treg differentiation by expressing PD-L1; Authors - Fei Gao