Phospho-ACC1 (Ser79) Polyclonal antibody, PBS Only

Phospho-ACC1 (Ser79) Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29119-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 29119-1-AP (same clone as 29119-1-PBS)

  Non-treated SH-SY5Y, phosphatase inhibitor treated and λ phosphatase treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 29119-1-AP (Phospho-ACC1 (Ser79) antibody) at dilution of 1:1000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with Vinculin antibody as loading control. This data was developed using the same antibody clone with 29119-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 29119-1-AP (same clone as 29119-1-PBS)

  Non-treated NIH/3T3, phosphatase inhibitor treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29119-1-AP (Phospho-ACC1 (Ser79) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29119-1-PBS in a different storage buffer formulation.

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Product Information

29119-1-PBS targets Phospho-ACC1 (Ser79) in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: acetyl-Coenzyme A carboxylase alpha
• Calculated Molecular Weight: 2383 aa, 275 kDa
• Observed Molecular Weight: 250 kDa
• GenBank Accession Number: BC137287
• Gene Symbol: Acetyl-CoA Carboxylase 1
• Gene ID (NCBI): 31
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ACC1 represents a key enzyme, as it is highly regulated by phosphorylation and allosteric regulation, providing a rapid adaptation to new micro-environmental conditions. AMPK phosphorylates acetyl CoA carboxylase (ACC), a rate-controlling step in the conversion of acetyl-CoA to malonyl CoA. This phosphorylation inhibits the activity of ACC, which results in decreased malonyl CoA levels. Additionally, two isoforms of ACC encoded by two different genes in mammalian cells have been described, ACC1 and ACC2. ACC1 is highly enriched in lipogenic tissues (liver and adipose), while ACC2 is mainly expressed in oxidative tissues (heart, skeletal muscle and liver). (PMID: 29056512, PMID: 16054041, PMID: 30816537)