Periostin Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Periostin Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A11A3

Cat no : 66491-1-PBS

Synonyms

OSF 2, OSF2, Osteoblast specific factor 2, PDLPOSTN, periostin, PN, POSTN, RP11 412K4.1

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Validation Data Gallery

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  WB analysis of BxPC-3 using 66491-1-Ig (same clone as 66491-1-PBS)

  BxPC-3, SGC-7901 and ROS1728 cells were subjected to SDS PAGE followed by western blot with 66491-1-Ig (Periostin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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  WB analysis of Neuro-2a using 66491-1-Ig (same clone as 66491-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66491-1-Ig (Periostin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 66491-1-Ig (same clone as 66491-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66491-1-Ig (Periostin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66491-1-Ig (same clone as 66491-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66491-1-Ig (Periostin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66491-1-Ig (same clone as 66491-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66491-1-Ig (Periostin antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66491-1-Ig (same clone as 66491-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66491-1-Ig (Periostin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66491-1-Ig (same clone as 66491-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66491-1-Ig (Periostin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 66491-1-Ig (same clone as 66491-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Periostin antibody (66491-1-Ig, Clone: 1A11A3 ) at dilution of 1:8000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 66491-1-Ig (same clone as 66491-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Periostin antibody (66491-1-Ig, Clone: 1A11A3 ) at dilution of 1:8000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66491-1-PBS targets Periostin in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Periostin fusion protein Ag14487
• Full Name: periostin, osteoblast specific factor
• Calculated Molecular Weight: 93 kDa
• Observed Molecular Weight: 85-90 kDa
• GenBank Accession Number: BC106710
• Gene Symbol: POSTN
• Gene ID (NCBI): 10631
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

Periostin (POSTN, PN), originally named as osteoblast-specific factor 2 (OSF-2), is a 90-kDa secreted protein which is now classified as a matricellular protein. It is present in a wide variety of normal adult tissues and fetal tissues, and has a role in bone, tooth and heart development and function. Studies show that periostin is overexpressed in a broad range of human cancer types, including lung, ovary, breast and colon cancers. Recent evidence reveals that periostin is expressed by fibroblasts in the normal tissue and in the stroma of the primary tumour, and it is required to allow cancer stem cell maintenance. The isoforms of periostin are between 83 and 93 kDa in mass and differ in their C-terminal sequences, characterized by individual presence or absence of cassette exons 17-21 (UniProtKB/Swiss-Prot, PMID: 21997759).