Periostin Polyclonal antibody

Periostin Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 27794-1-AP

Synonyms

PDLPOSTN, Osteoblast-specific factor 2, Osteoblast specific factor 2, OSF-2, OSF2

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Validation Data Gallery

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  IHC staining of human colon using 27794-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27794-1-AP (Periostin antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 27794-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27794-1-AP (Periostin antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 27794-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27794-1-AP (Periostin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 27794-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27794-1-AP (Periostin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 27794-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27794-1-AP (Periostin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27794-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27794-1-AP (Periostin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27794-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27794-1-AP (Periostin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 27794-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27794-1-AP (Periostin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 27794-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27794-1-AP (POSTN antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 27794-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27794-1-AP (POSTN antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human colon tissue, human colon cancer tissue, human breast cancer tissue, human liver cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27794-1-AP targets Periostin in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Periostin fusion protein Ag27145
• Full Name: periostin, osteoblast specific factor
• GenBank Accession Number: BC106710
• Gene Symbol: Periostin
• Gene ID (NCBI): 10631
• RRID: AB_2880975
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Periostin (POSTN, PN), originally named as osteoblast-specific factor 2 (OSF-2), is a 90-kDa secreted protein which is now classified as a matricellular protein. It is present in a wide variety of normal adult tissues and fetal tissues, and has a role in bone, tooth and heart development and function. Studies show that periostin is overexpressed in a broad range of human cancer types, including lung, ovary, breast and colon cancers. Recent evidence reveals that periostin is expressed by fibroblasts in the normal tissue and in the stroma of the primary tumour, and it is required to allow cancer stem cell maintenance.