Pericentrin Polyclonal antibody

Pericentrin Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27084-1-AP

Synonyms

KEN, Kendrin, KIAA0402, MOPD2, PCN, PCNT, PCNT2, PCNTB, PCTN2, pericentrin, Pericentrin B, SCKL4

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Validation Data Gallery

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  IHC staining of human thyroid cancer using 27084-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 27084-1-AP (Pericentrin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 27084-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 27084-1-AP (Pericentrin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 27084-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Pericentrin antibody (27084-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 27084-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Pericentrin antibody (27084-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human thyroid cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27084-1-AP targets Pericentrin in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Pericentrin fusion protein Ag25888
• Full Name: pericentrin
• Calculated Molecular Weight: 378 kDa
• GenBank Accession Number: AK093923
• Gene Symbol: Pericentrin
• Gene ID (NCBI): 5116
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PCNT, also named as KIAA0402, PCNT2, Pericentrin-B and Kendrin, is an integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. PCNT plays a role, together with DISC1, in the microtubule network formation. PCNT is a marker of centrosome. Defects in PCNT are the cause of microcephalic osteodysplastic primordial dwarfism type 2 (MOPD2).