Pericentrin Polyclonal antibody

Pericentrin Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, canine

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 22271-1-AP

Synonyms

PCNT, Pericentrin-B, Pericentrin B

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Validation Data Gallery

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  IHC staining of human intrahepatic cholangiocarcinoma using 22271-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 22271-1-AP (Pericentrin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MDCK using 22271-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using 22271-1-AP (Pericentrin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 22271-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Pericentrin antibody (22271-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of C2C12 using 22271-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Pericentrin antibody (22271-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 22271-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Pericentrin antibody (22271-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MDCK cells, HeLa cells, C2C12 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 3 publications below

Product Information

22271-1-AP targets Pericentrin in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, canine samples.

• Tested Reactivity: human, mouse, canine
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: pericentrin
• Calculated Molecular Weight: 378 kDa
• GenBank Accession Number: NM_006031
• Gene Symbol: Pericentrin
• Gene ID (NCBI): 5116
• RRID: AB_2879056
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PCNT, also named as KIAA0402, PCNT2, Pericentrin-B and Kendrin, is an integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. PCNT plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). PCNT is a marker of centrosome. Defects in PCNT are the cause of microcephalic osteodysplastic primordial dwarfism type 2 (MOPD2). The antibody is specific to PCNT, and it can recognize isoform 1 and 2.

Publications

Species	Application	Title
human	IF	Elife; De novo centriole formation in human cells is error-prone and does not require SAS-6 self-assembly.; Authors - Won-Jing Wang
human	IF	Cell Death Differ; Hornerin mediates phosphorylation of the polo-box domain in Plk1 by Chk1 to induce death in mitosis; Authors - Haiyu Song
human	IF	Cell Rep; Aurora A polyubiquitinates the BRCA1-interacting protein OLA1 to promote centrosome maturation; Authors - Zhenzhou Fang