Tested Applications
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MDCK cells, HeLa cells, C2C12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22271-1-AP targets Pericentrin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, canine samples.
| Tested Reactivity | human, mouse, canine |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | pericentrin |
| Calculated Molecular Weight | 378 kDa |
| GenBank Accession Number | NM_006031 |
| Gene Symbol | Pericentrin |
| Gene ID (NCBI) | 5116 |
| RRID | AB_2879056 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95613 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PCNT, also named as KIAA0402, PCNT2, Pericentrin-B and Kendrin, is an integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. PCNT plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). PCNT is a marker of centrosome. Defects in PCNT are the cause of microcephalic osteodysplastic primordial dwarfism type 2 (MOPD2). The antibody is specific to PCNT, and it can recognize isoform 1 and 2.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Pericentrin antibody 22271-1-AP | Download protocol |
| IHC protocol for Pericentrin antibody 22271-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Pericentrin Polyclonal antibody (Cat# 22271-1-AP) has a total of 5 citations. These appear in Cell Death & Differentiation, Cell Death & Disease, Cell Reports, Scientific Reports, and eLife. The associated research fields encompass mitotic regulation and cell death signaling, centrosome maturation and microtubule organization, primary cilia function in cardiovascular and cognitive biology, and de novo centriole biogenesis. Applications cited include immunofluorescence (IF) and Western blot (WB) in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Cell Death Differ Hornerin mediates phosphorylation of the polo-box domain in Plk1 by Chk1 to induce death in mitosis | ||
Cell Death Dis Cdk1-phosphorylated Nur77 accumulates at the centrosome during mitosis to regulate the Cep192-PLK1 signaling axis. | ||
Cell Rep Aurora A polyubiquitinates the BRCA1-interacting protein OLA1 to promote centrosome maturation | ||
Sci Rep Muscarinic acetylcholine receptor 3 localized to primary endothelial cilia regulates blood pressure and cognition | ||
Elife De novo centriole formation in human cells is error-prone and does not require SAS-6 self-assembly. |
