Pan-cadherin Polyclonal antibody

Pan-cadherin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 31313-1-AP

Synonyms

E-cadherin, CDH1, CD324, CAM 120/80, Cadherin-1

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Validation Data Gallery

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  WB analysis using 31313-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31313-1-AP (Pan-cadherin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 31313-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 31313-1-AP (Pan-cadherin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 31313-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 31313-1-AP (Pan-cadherin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 31313-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31313-1-AP (Pan-cadherin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 31313-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31313-1-AP (Pan-cadherin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 31313-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Pan-cadherin antibody (31313-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, MCF-7 cells, C6 cells, mouse brain tissue, mouse heart tissue
• Positive IHC detected in: mouse colon tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31313-1-AP targets Pan-cadherin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PTG
• Full Name: cadherin 1, type 1, E-cadherin (epithelial)
• Calculated Molecular Weight: 97 kDa
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: NM_004360
• Gene Symbol: E-cadherin
• Gene ID (NCBI): 999
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in tissue morphogenesis and the maintenance of normal tissue architecture (PMID: 23885883). The classic cadherin family members include E-, N-, P-, R-, and B-cadherins. The classic cadherins have ectodomains composed of five extracellular cadherin (EC) repeats, a single transmembrane region, and a cytoplasmic domain. Structural characterization indicated that the known cadherins are glycoproteins ranging in their apparent molecular weights between 120 and 140 kDa (PMID: 2077036). This polyclonal antibody is raised against a synthetic peptide corresponding to a conserved region of human E-Cadherin.