Pan Acetylation Monoclonal antibody

Pan Acetylation Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

chemical compound and More (5)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA, Acetylation assay

Conjugate

Unconjugated

Publications

41

CloneNo.

1D5F2

Reviews (1)

Cat no : 66289-1-Ig

Synonyms

Acetylation Lysine, Acetylation, Ac-lysine

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Validation Data Gallery

• 

  WB analysis of HeLa using 66289-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66289-1-Ig (Pan Acetylation Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 66289-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66289-1-Ig (Pan Acetylation antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of MDCK using 66289-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde) fixed MDCK cells using 66289-1-Ig (Pan Acetylation antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells, MDCK cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 33 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• CoIP: See 3 publications below

Product Information

66289-1-Ig targets Pan Acetylation in WB, IHC, IF/ICC, IP, CoIP, ELISA, Acetylation assay applications and shows reactivity with chemical compound samples.

• Tested Reactivity: chemical compound
• Cited Reactivity: human, mouse, rat, drosophila, rice
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: Pan Acetylation
• Observed Molecular Weight: 17 kDa
• RRID: AB_2881672
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Acetylation and De-acetylation are the important reaction in epigenetic. Many proteins, especially Histone, are with Acetylated of Lysine. This antibody is used to detect the Acetylation of Lysine. The 17 kDa in our detection is Acetylated Histone.

Publications

Species	Application	Title
mouse	WB,IF	Mol Cell; Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation; Authors - Chuanlong Wang
mouse	WB,IF	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li
Drosophila	WB	Dev Cell; HDAC6 Suppresses Age-Dependent Ectopic Fat Accumulation by Maintaining the Proteostasis of PLIN2 in Drosophila.; Authors - Yan Yan
human	WB	Cell Death Dis; RSL1D1 promotes the progression of colorectal cancer through RAN-mediated autophagy suppression.; Authors - Xunhua Liu
rice	Acetylation assay	Plant Biotechnol J; The rice RNase P protein subunit Rpp30 confers broad-spectrum resistance to fungal and bacterial pathogens.; Authors - Wei Li
human	WB	Theranostics; EBV(LMP1)-induced metabolic reprogramming inhibits necroptosis through the hypermethylation of the RIP3 promoter.; Authors - Feng Shi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xiaoyu (Verified Customer) (07-18-2024)

Gives a nice, strong and specific signal in Western Blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T

Pan Acetylation Antibody Western Blot validation (1:1000 dilution) in 293T (Cat no:66289-1-Ig)