PYGM-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

PYGM-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

9

Reviews (1)

Cat no : 19716-1-AP

Synonyms

PYGM, Myophosphorylase, EC:2.4.1.1

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Validation Data Gallery

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  WB analysis using 19716-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19716-1-AP (PYGM-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 19716-1-AP

  WB result of PYGM-Specific antibody (19716-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PYGM-Specific transfected HepG2 cells.

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  IHC staining of human skeletal muscle using 19716-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 19716-1-AP (PYGM-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 19716-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 19716-1-AP (PYGM-Specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 19716-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 19716-1-AP (PYGM-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 19716-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 19716-1-AP (PYGM-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 19716-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PYGM-Specific antibody (19716-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: mouse heart tissue, rat heart
• Positive IHC detected in: human skeletal muscle tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IHC: See 1 publications below

Product Information

19716-1-AP targets PYGM-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: phosphorylase, glycogen, muscle
• Calculated Molecular Weight: 97 kDa
• Observed Molecular Weight: 97-105 kDa
• GenBank Accession Number: NM_005609
• Gene Symbol: PYGM
• Gene ID (NCBI): 5837
• RRID: AB_10642009
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PYGM belongs to the glycogen phosphorylase family. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Defects in PYGM are the cause of glycogen storage disease type 5 (GSD5) which also known as McArdle disease. The antibody is specific to PYGM.

Publications

Species	Application	Title
mouse	WB	Nature; Glycogen metabolism links glucose homeostasis to thermogenesis in adipocytes.; Authors - Omer Keinan
human	WB	Int J Biol Sci; PYGL-mediated glucose metabolism reprogramming promotes EMT phenotype and metastasis of pancreatic cancer; Authors - Qian Ji
mouse	WB	Dis Model Mech; Absence of p.R50X Pygm read-through in McArdle disease cellular models.; Authors - Guillermo Tarrasó
mouse	WB	Cells; Proteomic Landscape and Deduced Functions of the Cardiac 14-3-3 Protein Interactome; Authors - Jia-Hua Qu
human	WB	Oncol Lett; Analysis of the expression, function and signaling of glycogen phosphorylase isoforms in hepatocellular carcinoma.; Authors - Lingyu Jiang
human	WB	Clin Proteomics; The different expression of glycogen phosphorylases in renal clear cell renal carcinoma and chromophobe renal carcinoma; Authors - Yang Lu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Patricia (Verified Customer) (02-25-2020)

Some non-specific bands appeared. Blocked in 5% skim milk and diluted antibody in TBS.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Ovarian cancer