PYGL Monoclonal antibody

• Featured Product
• KD/KO Validated

PYGL Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G12D8

Cat no : 66769-1-Ig

Synonyms

GSD6, phosphorylase, glycogen, liver, PYGL

---

Validation Data Gallery

• 

  WB analysis of HSC-T6 using 66769-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66769-1-Ig (PYGL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66769-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66769-1-Ig (PYGL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66769-1-Ig

  WB result of PYGL antibody (66769-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PYGL transfected HeLa cells.

---

Tested Applications

• Positive WB detected in: HSC-T6 cells, HeLa cells, HepG2 cells, L02 cells, SMMC-7721 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66769-1-Ig targets PYGL in WB, ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PYGL fusion protein Ag8550
• Full Name: phosphorylase, glycogen, liver
• Calculated Molecular Weight: 846 aa, 97 kDa
• Observed Molecular Weight: 97 kDa
• GenBank Accession Number: BC009895
• Gene Symbol: PYGL
• Gene ID (NCBI): 5836
• RRID: AB_2882115
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glycogen phosphorylase L (PYGL) is one of the gene related to hypoxia metabolism and was found to be up-regulated in head and neck squamous cell carcinomas (HNSCCs) and breast cancers. Glycogen synthases (GSs) and glycogen phosphorylases (GPs) catalyze the key steps of glycogen synthesis and breakdown, while glycogen phosphorylase has three isoforms: PYGM (muscle), PYGL (liver), and PYGB (brain) (PMID: 37063425, 9529348). High PYGL expression plays an independent role in predicting the poor prognosis of glioma patients (PMID: 34177761).