PYGB Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

PYGB Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 55380-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of A549 using 55380-1-AP (same clone as 55380-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 55380-1-AP (PYGB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55380-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 55380-1-AP (same clone as 55380-1-PBS)

  WB result of PYGB antibody (55380-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PYGB transfected MCF-7 cells. This data was developed using the same antibody clone with 55380-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 55380-1-AP (same clone as 55380-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 55380-1-AP (PYGB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55380-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 55380-1-AP (same clone as 55380-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 55380-1-AP (PYGB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55380-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 55380-1-AP (same clone as 55380-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 55380-1-AP (PYGB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55380-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 55380-1-AP (same clone as 55380-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 55380-1-AP (PYGB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55380-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-251 using 55380-1-AP (same clone as 55380-1-PBS)

  U-251 cells were subjected to SDS PAGE followed by western blot with 55380-1-AP (PYGB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55380-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 55380-1-AP (same clone as 55380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 55380-1-AP (PYGB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55380-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 55380-1-AP (same clone as 55380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 55380-1-AP (PYGB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55380-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 55380-1-AP (same clone as 55380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55380-1-AP (PYGB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55380-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 55380-1-AP (same clone as 55380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55380-1-AP (PYGB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55380-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 55380-1-AP (same clone as 55380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 55380-1-AP (PYGB antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55380-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 55380-1-AP (same clone as 55380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 55380-1-AP (PYGB antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55380-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 55380-1-AP (same clone as 55380-1-PBS)

  Immunofluorescent analysis of A549 cells using 55380-1-AP (PYGB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55380-1-PBS in a different storage buffer formulation.

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Product Information

55380-1-PBS targets PYGB in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: phosphorylase, glycogen; brain
• Calculated Molecular Weight: 97 kDa
• Observed Molecular Weight: 97-105 kDa
• GenBank Accession Number: NM_002862
• Gene Symbol: PYGB
• Gene ID (NCBI): 5834
• RRID: AB_11182159
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PYGB belongs to the glycogen phosphorylase family. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. This antibody is specific to PYGB. It has no cross reaction to PYGM and PYGL.