PYGB Polyclonal antibody

• Featured Product
• KD/KO Validated

PYGB Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

16

Reviews (1)

Cat no : 12075-1-AP

Synonyms

phosphorylase, glycogen; brain, GPBB, EC:2.4.1.1

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Validation Data Gallery

• 

  WB analysis of human brain using 12075-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12075-1-AP (PYGB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12075-1-AP

  WB result of PYGB antibody (12075-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PYGB transfected MCF-7 cells.

• 

  WB analysis of rat brain using 12075-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12075-1-AP (PYGB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12075-1-AP

  IP result of anti-PYGB (IP:12075-1-AP, 4ug; Detection:12075-1-AP 1:500) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of rat brain using 12075-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12075-1-AP (PYGB antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human normal colon using 12075-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12075-1-AP (PYGB antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 12075-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12075-1-AP (PYGB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 12075-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12075-1-AP (PYGB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human brain tissue, MCF-7 cells, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human normal colon, mouse heart tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 15 publications below
• IHC: See 2 publications below
• IP: See 2 publications below

Product Information

12075-1-AP targets PYGB in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PYGB fusion protein Ag2732
• Full Name: phosphorylase, glycogen; brain
• Calculated Molecular Weight: 843 aa, 97 kDa
• Observed Molecular Weight: 97 kDa
• GenBank Accession Number: BC017045
• Gene Symbol: PYGB
• Gene ID (NCBI): 5834
• RRID: AB_2174885
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Brain; C9orf72 expansion within astrocytes reduces metabolic flexibility in amyotrophic lateral sclerosis.; Authors - Scott P Allen
human	WB	Int J Biol Sci; PYGL-mediated glucose metabolism reprogramming promotes EMT phenotype and metastasis of pancreatic cancer; Authors - Qian Ji
human	WB	Mol Cell Proteomics; Multi-omics integration reveals the landscape of pro-metastasis metabolism in hepatocellular carcinoma.; Authors - Yongmei Li
		iScience; Using brain cell-type-specific protein interactomes to interpret neurodevelopmental genetic signals in schizophrenia; Authors - Yu-Han H Hsu
human	WB	Front Cell Dev Biol; Glycogen Phosphorylase B Is Regulated by miR101-3p and Promotes Hepatocellular Carcinoma Tumorigenesis.; Authors - Guangying Cui
mouse	WB	J Cell Mol Med; PMID: 21251210; Authors - Xi Lei L

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Patricia (Verified Customer) (02-25-2020)

works fine

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Ovarian cancer