PYCR2 Monoclonal antibody

PYCR2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G7D9

Cat no : 68488-1-Ig

Synonyms

FLJ54750, P5C reductase 2, P5CR 2, P5CR2, PYCR2

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Validation Data Gallery

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  WB analysis using 68488-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68488-1-Ig (PYCR2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68488-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68488-1-Ig (PYCR2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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Tested Applications

• Positive WB detected in: Jurkat cells, LNCaP cells, K-562 cells, THP-1 cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:20000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68488-1-Ig targets PYCR2 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PYCR2 fusion protein Ag10923
• Full Name: pyrroline-5-carboxylate reductase family, member 2
• Calculated Molecular Weight: 320 aa, 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC020553
• Gene Symbol: PYCR2
• Gene ID (NCBI): 29920
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PYCR2, also named as P5CR2, belongs to the pyrroline-5-carboxylate reductase family. It catalyses the reaction: L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H. This antibody may have cross reaction to PYCR1 due to the high homology.