PYCR1 Monoclonal antibody, PBS Only

PYCR1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H2G1

Cat no : 66510-1-PBS

Synonyms

P5C, P5C reductase 1, P5CR, P5CR 1, PIG45, PP222, PYCR, PYCR1

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Validation Data Gallery

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  WB analysis using 66510-1-Ig (same clone as 66510-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66510-1-Ig (PYCR1 antibody) at dilution of 1:3000 incubated at room temperature for 1 hour. This data was developed using the same antibody clone with 66510-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66510-1-Ig (same clone as 66510-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66510-1-Ig (PYCR1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66510-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66510-1-Ig (same clone as 66510-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66510-1-Ig (PYCR1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66510-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66510-1-Ig (same clone as 66510-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PYCR1 antibody (66510-1-Ig, Clone: 1H2G1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66510-1-PBS in a different storage buffer formulation.

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Product Information

66510-1-PBS targets PYCR1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: PYCR1 fusion protein Ag17919
• Full Name: pyrroline-5-carboxylate reductase 1
• Calculated Molecular Weight: 319 aa, 33.8 kDa
• Observed Molecular Weight: 33 kDa, 35 kDa
• GenBank Accession Number: BC022244
• Gene Symbol: PYCR1
• Gene ID (NCBI): 5831
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PYCR1,also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is a housekeeping enzyme that catalyzes the last step in proline biosynthesis. PYCR1 can utilize both NAD and NADP, but has higher affinity for NAD. It is involved in the cellular response to oxidative stress. Mutation in PYCR1 will cause ARCL type II(ARCL2B ). Some mutation will cause DeBarsy syndrome (DBS) which is characterized by progeroid features, ophthalmological abnormalities, intrauterine growth retardation, and cutis laxa.