ASC/TMS1 Recombinant antibody

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  WB analysis using 83858-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83858-3-RR (PYCARD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 83858-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83858-3-RR (ASC/TMS1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 83858-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83858-3-RR (ASC/TMS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83858-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83858-3-RR against Mouse PYCARD were performed. The affinity constant is 3.58 nM.

ASC/TMS1 Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Cat No. 83858-3-RR

Clone No.240908D5

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Synonyms

Pycard, PYD and CARD domain containing, masc, CARD5, Asc

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Tested Applications

• Positive WB detected in: J774A.1 cells, mouse lung tissue, mouse thymus tissue, mouse spleen tissue
• Positive IHC detected in: mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83858-3-RR targets ASC/TMS1 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ASC/TMS1 fusion protein Ag32519
• Full Name: PYD and CARD domain containing
• Calculated Molecular Weight: 21KD
• Observed Molecular Weight: 21-25 kDa
• GenBank Accession Number: NM_023258
• Gene Symbol: Pycard
• Gene ID (NCBI): 66824
• RRID: AB_3671442
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9EPB4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PYCARD (PYD and CARD domain containing), also known as ASC and CARD5. The calculated molecular weight of PYCARD is 21 kDa. It is located in cytosol, extracellular region and nucleus. PYCARD is also expressed in several structures, including alimentary system; brain; heart; hemolymphoid system gland; and urinary system. The gene enables identical protein binding activity and protein dimerization activity. Involved in several processes, including activation of cysteine-type endopeptidase activity; positive regulation of cytokine production; and regulation of defense response. It acts upstream of or within several processes, including defense response to Gram-positive bacterium; positive regulation of macromolecule metabolic process; and regulation of autophagy. It is also a part of inflammasome complex. Finally, it orthologous to human PYCARD (PYD and CARD domain containing).