PURA Polyclonal antibody

• Featured Product
• KD/KO Validated

PURA Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, RIP, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 17733-1-AP

Synonyms

PUR ALPHA, PUR1, PURA, PURALPHA

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Validation Data Gallery

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  WB analysis using 17733-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17733-1-AP (PURA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17733-1-AP

  WB result of PURA antibody (17733-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PURA transfected HepG2 cells.

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Tested Applications

• Positive WB detected in: A549 cells, HepG2 cells, Hela cells, U-87 MG cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• RIP: See 1 publications below

Product Information

17733-1-AP targets PURA in WB, IP, IF, RIP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PURA fusion protein Ag12173
• Full Name: purine-rich element binding protein A
• Calculated Molecular Weight: 322 aa, 35 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC036087
• Gene Symbol: PURA
• Gene ID (NCBI): 5813
• RRID: AB_2173875
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PURA, also named as Transcriptional activator protein Pur-alpha, is a 322 amino acid protein, which belongs to the PUR DNA-binding protein family and can form homodimer or heterodimer with PURB and heterotrimer with PURB and YBX1/Y-box protein 1. PURA is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene and may play a role in the initiation of DNA replication and in recombination.

Publications

Species	Application	Title
human	RIP	Nucleic Acids Res; Corrigendum to article "Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation''.; Authors - Ruibing Chen
human	WB,IHC,IF,IP	Cancer Res; LncRNA AGPG confers endocrine resistance in breast cancer by promoting E2F1 activity; Authors - Shiyi Yu
human	WB,IP	EBioMedicine; Genome-wide CRISPR/Cas9 screening identifies a targetable MEST-PURA interaction in cancer metastasis; Authors - Wen Wen Xu ; KD Validated
human	WB	Nucleic Acids Res; Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation.; Authors - Ruibing Chen
rat	WB	Front Mol Neurosci; YAP and TAZ Regulate Cc2d1b and Purβ in Schwann Cells.; Authors - Belin Sophie
rat	WB	Acta Histochem; Protective effects of astaxanthin on subarachnoid hemorrhage-induced early brain injury: Reduction of cerebral vasospasm and improvement of neuron survival and mitochondrial function.; Authors - Yanbin Wang